Critical sites for the interaction between IL-2R gamma and JAK3 and the following signaling
文献类型：期刊论文
| 作者 | Tang, W; Hue, HR; Zhu, JF; Ji, HB; Zou, WG; Xu, LF; Sun, LY; Zheng, ZC; Theze, J; Liu, XY |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2001 |
| 卷号 | 283期号:3页码:598-605 |
| 关键词 | IL-2 R gamma JAK3 yeast two-hybrid system signal transduction |
| 通讯作者 | Liu, XY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 YueYang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | JAK3 is the only known protein tyrosine kinase associating with IL-2R gamma. This interaction is supposed to be very important to IL-2 signaling. In order to identify the critical residues for these two molecular interactions and the following signal events, various mutants of gammac and JAK3 were constructed on the basis of computer analysis. The direct interaction was determined via the yeast two-hybrid system, while the signaling was analyzed with reporter genes under the control of the c-fos, c-myc, or tnf-beta promoters, respectively. Results showed that there are two key sites on gammac involved in this interaction and the following signal transduction: the critical one is E327 via electrostatic interaction, the other is L293 via hydrophobic interaction. As to JAK3, the data indicated that Y100 is important for the interaction with gammac. These results also document that the requirement for interaction between gammac and JAK3 is different to activate different signaling pathways mediated by gammac, such as c-fos, c-myc, and JAK-STAT. (C) 2001 Academic Press. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | RECEPTOR-GAMMA-CHAIN ; MEMBRANE-PROXIMAL REGION ; IL-2 RECEPTOR ; BETA-CHAIN ; FUNCTIONAL COMPONENT ; KINASE ACTIVATION ; PROTEIN-KINASE ; C-MYC ; TRANSDUCTION ; INDUCTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000168927600010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2627]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Tang, W,Hue, HR,Zhu, JF,et al. Critical sites for the interaction between IL-2R gamma and JAK3 and the following signaling[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2001,283(3):598-605. |
| APA | Tang, W.,Hue, HR.,Zhu, JF.,Ji, HB.,Zou, WG.,...&Liu, XY.(2001).Critical sites for the interaction between IL-2R gamma and JAK3 and the following signaling.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,283(3),598-605. |
| MLA | Tang, W,et al."Critical sites for the interaction between IL-2R gamma and JAK3 and the following signaling".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 283.3(2001):598-605. |
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