Expression of human angiostatin in Pichia pastoris and the detection of its anti-angiogenic activity
文献类型：期刊论文
| 作者 | Xin, L; Zhang, L; Xu, R; Zhang, Q; Ye, Q; Li, ZP; Gan, RB |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 33期号:3页码:291-295 |
| 关键词 | angiostatin Pichia pastoris multiple copy integration inhibit endothelial cell |
| 通讯作者 | , |
| 英文摘要 | Human angiostatin cDNA was amplified from human hepatoma cell line HepG2 using RT-PCR and was cloned into pPIC9K vector. Recombinant Pichia pastoris strain with 4 copies of angiostatin gene was obtained. Recombinant protein was purified by lysine-affinity Sepharose column and the finally purified angiostatin was 25 mg/L, higher than previously reported 17 mg/L. Amino acid sequence analysis revealed the identity of our protein the same with that previously reported. Recombinant angiostatin inhibited specifically the proliferation of bovine aortic endothelial cell stimulated by bFGF, with ED50 being about 3 mg/L. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ENDOTHELIAL-CELLS ; PLASMINOGEN ; INHIBITOR ; SUPPRESSION ; CARCINOMA |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000168839400007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2629]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xin, L,Zhang, L,Xu, R,et al. Expression of human angiostatin in Pichia pastoris and the detection of its anti-angiogenic activity[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(3):291-295. |
| APA | Xin, L.,Zhang, L.,Xu, R.,Zhang, Q.,Ye, Q.,...&Gan, RB.(2001).Expression of human angiostatin in Pichia pastoris and the detection of its anti-angiogenic activity.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(3),291-295. |
| MLA | Xin, L,et al."Expression of human angiostatin in Pichia pastoris and the detection of its anti-angiogenic activity".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.3(2001):291-295. |
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