Phages displaying peptides with beta turn conformation
文献类型：期刊论文
| 作者 | Li, JD; Tang, WG; Wang, KY |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 33期号:3页码:335-339 |
| 关键词 | phage displayed peptide library beta turn |
| 通讯作者 | , |
| 英文摘要 | Peptide CX(2)GPX(4)C, which was supposed to adopt a typical beta turn conformation, was displayed on the surface of filamentous phages by fusing to the N-terminus of minor coat protein g3p. The diversity of this library was 1.04 x 10(8). Sequences from 19 randomly selected phage clones indicated that the distribution of nucleotides and amino acids paralleled the expected frequency. The combined index of hydropathy and isoelectric point of these 19 peptides were randomly distributed over wide ranges. The enrichment was obtained after 3 rounds of screening with monoclonal antibody 12CA5. And the results of phage ELISA and competitive ELISA showed that the 15 individual phage clones randomly selected from the third round clution specifically bound to the antigen-binding site of the antibody. This binding was abolished after destroying of the conformation. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | FILAMENTOUS PHAGE ; PROTEIN HORMONE ; LIBRARY ; RECEPTOR ; IDENTIFICATION ; ANTIBODY ; AGONIST ; LIGANDS ; EPITOPE ; POTENT |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000168839400015 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2630]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, JD,Tang, WG,Wang, KY. Phages displaying peptides with beta turn conformation[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(3):335-339. |
| APA | Li, JD,Tang, WG,&Wang, KY.(2001).Phages displaying peptides with beta turn conformation.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(3),335-339. |
| MLA | Li, JD,et al."Phages displaying peptides with beta turn conformation".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.3(2001):335-339. |
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