Identification of putative downstream genes of Oct-4 by suppression-subtractive hybridization
文献类型：期刊论文
| 作者 | Du, ZW; Cong, HC; Yao, Z |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2001 |
| 卷号 | 282期号:3页码:701-706 |
| 关键词 | Oct-4 downstream gene suppression-subtractive hybridization embryonic stem cell |
| 通讯作者 | Du, ZW (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | As a step toward understanding how toti/pluripotence is maintained by Oct-4, we have first constructed a cell model with differentially expressed Oct-4 in embryonic stem cells, and then used suppression-subtractive hybridization (SSH) method to identify the downstream genes of Oct-4. Among the 384 clones we screened, 40 clones were detected as differentially expressed genes with colony hybridization, and 13 clones were confirmed as the putative downstream genes of Oct-4 by Northern blot analysis. Sequencing showed 12 different genes, 8 known genes (Oct-4, Rex-1, Sox-2, Creatine kinase B, Makorin 1, Importin beta, Histone H2A.Z, Ribosomal protein S7) and 4 new genes. Except Oct-4 and Rex-1, the other genes have not been reported to be regulated by Oct-4, These results showed that SSH provides a very efficient means to identify the downstream genes of transcription factor. Some known genes identified may provide new insight of the function of Oct-4 in stem cells. (C) 2001 Academic Press. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | EMBRYONIC STEM-CELLS ; TRANSCRIPTION FACTOR ; EXPRESSION ; DIFFERENTIATION ; SOX-2 ; PROMOTER ; ENHANCER ; BINDING ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000168092000008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2632]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Du, ZW,Cong, HC,Yao, Z. Identification of putative downstream genes of Oct-4 by suppression-subtractive hybridization[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2001,282(3):701-706. |
| APA | Du, ZW,Cong, HC,&Yao, Z.(2001).Identification of putative downstream genes of Oct-4 by suppression-subtractive hybridization.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,282(3),701-706. |
| MLA | Du, ZW,et al."Identification of putative downstream genes of Oct-4 by suppression-subtractive hybridization".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 282.3(2001):701-706. |
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