Human xenoreactivity is reduced in mice bearing porcine antisense alpha(1,3) galactosyltransferase cDNA
文献类型：期刊论文
| 作者 | Ma, YH; Zhou, XG; Hu, JH; Fei, J; Xia, GH; Guo, LH |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 22期号:3页码:231-238 |
| 关键词 | epitopes galactosyltransferases heterologous transplantation fluorescent antibody technique antisense DNA reverse polymerase chain reaction Southern blotting swine |
| 通讯作者 | Guo, LH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | AIM: To explore the effect of antisense alpha (1,3) galactosyltransferase alpha (1,3) GT cDNA on production of Gal a (1,3) Gal(Gal epitope) xenoantigen in vivo. METHODS: Transgenic mice bearing the porcine antisense all, 3) GT cDNA (nt 1 alpha - 640) were generated by pronuclei microinjection method. The integration of transgene was identified by PCR and Southern-blot analysis. The expression of murine a(1,3) GT was characterized by RT-PCR. Morphology of the spleen was examined by histological technique. Gal epitope was detected by immunofluorescent analysis. Binding of human natural xenoantibodies (IgM and IgG) and complement (C3c) to cells from mice was determined by flow cytometric assay. RESULTS: Transgenic mice bearing the porcine antisense a(1,3) GT cDNA were born healthy and developed normally. However, necrosis occurred in the spleen of some mice heterozygous for transgene. Cell surface Gal epitope in transgenic heterozygotes was evidently reduced. Substantially less (30 % - 60 %) xenoantibodies in human serum bound to cells from a variety of tissues of transgenic heterozygotes compared with wild-type controls. Consequentially, human complement activation on cells from these mice was reduced by 40 % - 50 %. CONCLUSION: Human xenoreactivity could be effectively reduced by inhibiting the expression of a (1,3) galactosyltransferase with an antisense gene. |
| 学科主题 | Chemistry; Pharmacology & Pharmacy |
| 类目[WOS] | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 关键词[WOS] | DECAY-ACCELERATING FACTOR ; TRANSGENIC MICE ; EXPRESSION ; ANTIBODIES ; CELLS ; PIG ; ALPHA-1,3-GALACTOSYLTRANSFERASE ; EPITOPES ; GENE ; XENOGRAFTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000167403800006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2633]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ma, YH,Zhou, XG,Hu, JH,et al. Human xenoreactivity is reduced in mice bearing porcine antisense alpha(1,3) galactosyltransferase cDNA[J]. ACTA PHARMACOLOGICA SINICA,2001,22(3):231-238. |
| APA | Ma, YH,Zhou, XG,Hu, JH,Fei, J,Xia, GH,&Guo, LH.(2001).Human xenoreactivity is reduced in mice bearing porcine antisense alpha(1,3) galactosyltransferase cDNA.ACTA PHARMACOLOGICA SINICA,22(3),231-238. |
| MLA | Ma, YH,et al."Human xenoreactivity is reduced in mice bearing porcine antisense alpha(1,3) galactosyltransferase cDNA".ACTA PHARMACOLOGICA SINICA 22.3(2001):231-238. |
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