Investigating the function of Akt by tet-off inducible expression system
文献类型：期刊论文
| 作者 | Zhai, QW; Ji, HB; Zheng, ZC; Sun, LY; Liu, XY |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2001 |
| 卷号 | 46期号:3页码:222-225 |
| 关键词 | inducible expression system Akt apoptosis |
| 通讯作者 | Zheng, ZC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | A tet-off inducible cell line named BBT derived from BA/F3 beta cell line was constructed and the effect of this inducible expression system was significant when detected by tet-off responded luciferase reporter gene assay. Then tet-off responded Akt expression plasmid was transfected into BET cells, and the stable cell lines were screened. The result of Northern blot showed that the expression of akt was significantly inducible. The clone with the best inducible effect was selected and named BBA for investigating the function of Akt. We found that Akt could significantly inhibit zinc-induced decrease of cell viability when assayed by MTT method. And the flow cytometric analysis showed that Akt could markedly repress zinc-induced apoptosis. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | GENE-EXPRESSION ; PHOSPHOINOSITIDE 3-KINASE ; MAMMALIAN-CELLS ; APOPTOSIS ; PHOSPHORYLATION ; REPRESSOR ; SURVIVAL ; PATHWAY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000167179800014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2635]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhai, QW,Ji, HB,Zheng, ZC,et al. Investigating the function of Akt by tet-off inducible expression system[J]. CHINESE SCIENCE BULLETIN,2001,46(3):222-225. |
| APA | Zhai, QW,Ji, HB,Zheng, ZC,Sun, LY,&Liu, XY.(2001).Investigating the function of Akt by tet-off inducible expression system.CHINESE SCIENCE BULLETIN,46(3),222-225. |
| MLA | Zhai, QW,et al."Investigating the function of Akt by tet-off inducible expression system".CHINESE SCIENCE BULLETIN 46.3(2001):222-225. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。