Function of GATA transcription factors in hydroxyurea-induced HEL cells
文献类型：期刊论文
| 作者 | Zhang, SB; He, QY; Zhao, H; Gui, CY; Jiang, C; Qian, RL |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2001 |
| 卷号 | 11期号:4页码:301-310 |
| 关键词 | GATA transcription factors human beta-globin gene HEL cells hydroxyurea |
| 通讯作者 | Qian, RL (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yueyang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | HEL cells, a human erythroleukemia cell line, mainly express the fetal (gamma )globin gene and trace amount of the embryonic (epsilon )globin gene, but not adult (beta) globin gene. Here we show that hydroxyurea (HU) can induce HEL cells to express adult (beta) globin gene and lead these cells to terminal differentiation. Results showed in Gel mobility shift assays that GATA factors could specifically bind to the regulatory elements of human beta- globin gene, including the proximal regulatory element (the beta- promoter) and the distal regulatory elements (the DNase I hypersensitive sites in the LCR, HS2-HS4 core sequences), However, the DNA binding patterns of GATA factors were quite different between HU-induced and uninduced HEL cells, Western-blot analysis of nuclear extracts from both the uninduced and HU- induced HEL cells revealed that the level of GATA-2 transcription factor decreased, whereas the level of GATA-1 transcription factor increased following the time of hydroxyurea induction. Furthermore, using RT-PCR analysis the expression of human beta -globin gene in HU-induced HEL cells could be blocked again when HEL cells were incubated in the presence of antisense oligonucleotides for hGATA-1, suggesting that the upregulation of hGATA-1 transcription factor might be critical for the expression of human beta- globin gene in HU-induced HEL cells. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | GLOBIN GENE-EXPRESSION ; ERYTHROID-DIFFERENTIATION ; BINDING PROTEINS ; MESSENGER-RNA ; THALASSEMIA ; INITIATION ; CHROMATIN ; LINEAGES ; LACKING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000173058800008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2641]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, SB,He, QY,Zhao, H,et al. Function of GATA transcription factors in hydroxyurea-induced HEL cells[J]. CELL RESEARCH,2001,11(4):301-310. |
| APA | Zhang, SB,He, QY,Zhao, H,Gui, CY,Jiang, C,&Qian, RL.(2001).Function of GATA transcription factors in hydroxyurea-induced HEL cells.CELL RESEARCH,11(4),301-310. |
| MLA | Zhang, SB,et al."Function of GATA transcription factors in hydroxyurea-induced HEL cells".CELL RESEARCH 11.4(2001):301-310. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。