Expression, purification and preliminary clinical use of recombinant HBsAg GST-PreS1(21-47 aa) fusion proteins
文献类型：期刊论文
| 作者 | Wei, J; Liu, XJ; Li, GD; Wang, Y; Zhang, ZC; Wang, YQ; Lu, ZM |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 33期号:4页码:379-385 |
| 关键词 | PreS1(21-47 aa) antigen peptide GST-PreS1(21-47 aa) fusion protein anti-PreS1(21-47 aa) antibody biotin labeled protein A ELISA |
| 通讯作者 | , |
| 英文摘要 | Expression plasmids pGEXSI and pGEXSII containing one copy and two orderly joined copies of PreS1 (21-47 aa) DNA fragment, respectively, were constructed. GST-PreS1 (21-47 aa) and GST-2 X PreS1 (21-47 aa) fusion proteins were highly expressed in E. Coli TG1, induced by IPTG. The expression level of GST-PreS1 (21-47 aa) was about 30 % of total soluble proteins in the lysate of expression bacteria, and GST-2 X PreS1 (21-47 aa) was about 15 % of total soluble proteins, as estimated by SDS-PAGE. 50 mg GST-PreS1 (21-47 aa) or 20 mg GST-2 X PreS1 (21-47 aa) with purity over 90 % was obtained I respectively, from I L culture by using affinity chromatography of glutathione-Sepharose 4B. Direct ELISA results showed that antigenicity of GST-2 X PreS1 (21-47 aa) was better than GST-PreS1 (21-47 aa) and synthetic peptide. Using GST-2 X PreS1 (21-47 aa) as coated antigen, a sensitive indirect ELISA for detection of anti-PreS1 (21-47 aa) antibody, based on protein A-biotin and streptavidin-HRP, was established. The results from 99 sera samples of hepatitis B patients showed that anti-PreS1 (21-47 aa) antibody was detected in nearly half of acute hepatitis B patients during recovery, but it was detected only in a few chronic hepatitis patients. Clinical follow-up study suggested that appearance of anti-PreS1 (21-47 aa) was related to the course of the disease and recovery of patients. Detection system established in the study is promising for clinical application. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | HEPATITIS-B VIRUS ; RECEPTOR-BINDING SITE ; SURFACE-ANTIGEN ; MONOCLONAL-ANTIBODIES ; PICHIA-PASTORIS ; INFECTION ; PRES1 ; IMMUNIZATION ; SPECIFICITY ; EPITOPES |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000170070500004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2646]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wei, J,Liu, XJ,Li, GD,et al. Expression, purification and preliminary clinical use of recombinant HBsAg GST-PreS1(21-47 aa) fusion proteins[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(4):379-385. |
| APA | Wei, J.,Liu, XJ.,Li, GD.,Wang, Y.,Zhang, ZC.,...&Lu, ZM.(2001).Expression, purification and preliminary clinical use of recombinant HBsAg GST-PreS1(21-47 aa) fusion proteins.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(4),379-385. |
| MLA | Wei, J,et al."Expression, purification and preliminary clinical use of recombinant HBsAg GST-PreS1(21-47 aa) fusion proteins".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.4(2001):379-385. |
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