Unfolding of recombinant single-chain insulin in denaturants containing thiol reagents
文献类型：期刊论文
| 作者 | Guo, ZY; Qiao, ZS; Feng, YM |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 33期号:4页码:431-436 |
| 关键词 | recombinant single-chain insulin denaturation unfolding disulfide refolding |
| 通讯作者 | , |
| 英文摘要 | Recombinant single-chain insulin (PIP) contains three disulfide bonds. In the presence of denaturants and thiol reagents, the native structure of PIP was disturbed and its native disulfides were shuffled to form a mixture of scrambled isomers which have different degrees of unfolding. In this paper the unfolding degrees of PIP in urea or guanidine hydrochloride containing 0.2 mmol/L 2-mercaptoethanol was analyzed by reverse-phase HPLC and far-UV circular dichroism (CD). The peptide mapping of PIP scrambles demonstrated that PIP had shuffled its native disulfides under the condition we used. Among others, a major non-natural PIP disulfide isomer was purified and its refolding in vitro was investigated. These results show that PIP has only one thermodynamically stable disulfide linkage, and the non-natural disulfide isomers could refold in vitro efficiently to from native PIP. On basis of these, the differences between PIP, IGF-I and insulin on unfolding and refolding were discussed. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | GROWTH FACTOR-I ; SCRAMBLED HIRUDINS ; FOLDING PATHWAY ; A-CHAIN ; PROTEIN ; STABILITY |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000170070500013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2650]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Guo, ZY,Qiao, ZS,Feng, YM. Unfolding of recombinant single-chain insulin in denaturants containing thiol reagents[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(4):431-436. |
| APA | Guo, ZY,Qiao, ZS,&Feng, YM.(2001).Unfolding of recombinant single-chain insulin in denaturants containing thiol reagents.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(4),431-436. |
| MLA | Guo, ZY,et al."Unfolding of recombinant single-chain insulin in denaturants containing thiol reagents".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.4(2001):431-436. |
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