Identification of epitopes of trichosanthin by phage peptide library
文献类型：期刊论文
| 作者 | Zhu, ZY; Yeh, M; Sun, B |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2001 |
| 卷号 | 282期号:4页码:921-927 |
| 关键词 | trichosanthin antigen epitope phage display peptide library site-directed mutation |
| 通讯作者 | Sun, B (reprint author), Chinese Acad Sci, Lab Mol Immunol, Shanghai Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yueyang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | The phage displayed random peptide library has recently emerged as a powerful technique for analyzing Ab-Ag interactions. In this study, the method was employed to identify epitopes of trichosanthin. Two monoclonal Abs (4B5, 2E9) which recognized different epitopes of trichosanthin (TCS) were selected and a phage-peptide library with nine amino acids (9 aa) was used to screen the positive phage clones that have high affinity to the mAbs. Two groups of phage clones that carried peptide-specific binding to mAbs were identified by the screen. The identified phage clones carried peptide-specific binding to 4B5 and 2E9 mAbs were immunized in mice. To evaluate mimotope of selected phages, the specific binding activity to TCS was measured in the serum from phage-immunized mice. They all showed positive results. The conserved interaction motifs were deduced from the peptide sequences of each group of selected phage clones. When compared the motif sequence with the sequence of TCS, it was predicted that 4B5-corresponding epitope was located at 27-37 aa of TCS protein and 2E9-corresponding epitope was located at 41-48 aa of TCS. The predicted sequence of 4B5-corresponding epitope was further con firmed by site-directed mutation of TCS protein. The data showed that the expressed TCS protein mutated in 4B5-corresponding epitope was unable to bind 4B5 mAb. The results suggested that the phage display peptide library is useful to identify Ag epitopes and to raise Ab in disease diagnosis and treatment. (C) 2001 Academic Press. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ANTIBODY ; MIMOTOPES ; SELECTION ; LIGANDS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000168258400012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2652]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhu, ZY,Yeh, M,Sun, B. Identification of epitopes of trichosanthin by phage peptide library[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2001,282(4):921-927. |
| APA | Zhu, ZY,Yeh, M,&Sun, B.(2001).Identification of epitopes of trichosanthin by phage peptide library.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,282(4),921-927. |
| MLA | Zhu, ZY,et al."Identification of epitopes of trichosanthin by phage peptide library".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 282.4(2001):921-927. |
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