Transgenic mice ubiquitously expressing human Fas ligand develop a slight form of graft-versus-host-like disease
文献类型：期刊论文
| 作者 | Ma, YH; Fei, J; Hu, JH; Zhou, XG; Xia, GH; Guo, LH |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 22期号:4页码:311-319 |
| 关键词 | Fas ligand graft vs Host disease transgenic mice flow cytometry reverse transcriptase polymerase chain reaction antisense DNA fluorescent antibody technique |
| 通讯作者 | Guo, LH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | AIM: To construct transgenic mice bearing human Fas ligand (FasL/CD95L) cDNA, and further explore the physiological effects of ubiquitous expression of Fast on such animals. METHODS: Transgenic mice were produced by pronuclei microinjection method. Integration and transmission of transgene were identified by nest-PCR and Southern-blot analysis. Level of Fast mRNA was evaluated by semi-quantitative RT-PCR analysis. Fast protein was detected by immunofluorescence analysis. Morphological alterations in tissues were analyzed by histological examination. The percentage of alpha betaT cells in the spleen was determined by flow cytometry analysis. RESULTS: Two independent founder mice bearing human Fast cDNA under the control of CMV promoter were generated healthily. Human Fast was moderately expressed in the majority of tissues examined in F1 heterozygotic mice. Although developing normally, adult transgenic mice exhibited a slight form of graft-versus-host (GVH)-like disease characterized by many morphological abnormalities occurring locally in the spleen, testis, lung and liver. In addition, the percentage of alpha betaT cells in the spleen was respectively decreased approximately by 32 % and 24 % in two independent transgenic lines, relative to wild-type mice. CONCLUSION: U-biquitous expression of Fas ligand can lead to slight GVH-like disease. |
| 学科主题 | Chemistry; Pharmacology & Pharmacy |
| 类目[WOS] | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 关键词[WOS] | CONFER IMMUNE PRIVILEGE ; MEDIATED CYTOTOXICITY ; LYMPHOCYTE APOPTOSIS ; UP-REGULATION ; CD95 LIGAND ; CELLS ; REJECTION ; ACTIVATION ; ISLETS ; MECHANISM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000168072800004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2653]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ma, YH,Fei, J,Hu, JH,et al. Transgenic mice ubiquitously expressing human Fas ligand develop a slight form of graft-versus-host-like disease[J]. ACTA PHARMACOLOGICA SINICA,2001,22(4):311-319. |
| APA | Ma, YH,Fei, J,Hu, JH,Zhou, XG,Xia, GH,&Guo, LH.(2001).Transgenic mice ubiquitously expressing human Fas ligand develop a slight form of graft-versus-host-like disease.ACTA PHARMACOLOGICA SINICA,22(4),311-319. |
| MLA | Ma, YH,et al."Transgenic mice ubiquitously expressing human Fas ligand develop a slight form of graft-versus-host-like disease".ACTA PHARMACOLOGICA SINICA 22.4(2001):311-319. |
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