Overexpression of gamma-aminobutyric acid transporter subtype I leads to cognitive deterioration in transgenic mice
文献类型：期刊论文
| 作者 | Ma, YH; Zhou, XG; Duan, SH; Hu, JH; Lu, BF; Yu, Y; Mei, ZT; Fei, J; Guo, LH |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 22期号:4页码:340-348 |
| 关键词 | carrier proteins gene expression cognition learning memory synapses transgenic mice |
| 通讯作者 | Guo, LH (reprint author), Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | AIM: To explore the physiological role of gamma -aminobutyric acid transporter subtype I (GAT1) in cognition. METHODS: Transgenic mice were produced by pronuclei microinjection method. Integration of transgene was identified by Southern-blot and PCR analysis in various generations. Level of GAT1 mRNA in a variety of tissues was evaluated by semi-quantitative RT-PCR analysis. GAT1 protein was detected by immunofluorescence and histochemistry analysis. Associative learning capacity was analyzed by conditioned avoidance task. Memory retention was assessed by novel object recognition test. Morphology of synaptosomes was examined by electron microscope. RESULTS: Four independent founder mice bearing various copies of transgene were generated. GAT1 was evidently overexpressed at both mRNA and protein level in a variety of tissues from transgenic mice. In comparision with wild-type mice, transgenic mice exhibited significantly declined associative learning capacity (P < 0.01) and decreased memory retention (P < 0.01 in 1-h-retention, and P < 0.05 in 1-d-retention). In addition, the amount of asymmetric synapses in the brain of transgenic mice was reduced approximately by 24 %, relative to wild-type mice. CONCLUSION: Overexpression of GAT1 in mice results in cognitive deterioration, indicating that the alteration in the expression of |
| 学科主题 | Chemistry; Pharmacology & Pharmacy |
| 类目[WOS] | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 关键词[WOS] | GABA ; EXPRESSION ; MEMORY ; BRAIN ; GENE ; RAT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000168072800008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2654]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ma, YH,Zhou, XG,Duan, SH,et al. Overexpression of gamma-aminobutyric acid transporter subtype I leads to cognitive deterioration in transgenic mice[J]. ACTA PHARMACOLOGICA SINICA,2001,22(4):340-348. |
| APA | Ma, YH.,Zhou, XG.,Duan, SH.,Hu, JH.,Lu, BF.,...&Guo, LH.(2001).Overexpression of gamma-aminobutyric acid transporter subtype I leads to cognitive deterioration in transgenic mice.ACTA PHARMACOLOGICA SINICA,22(4),340-348. |
| MLA | Ma, YH,et al."Overexpression of gamma-aminobutyric acid transporter subtype I leads to cognitive deterioration in transgenic mice".ACTA PHARMACOLOGICA SINICA 22.4(2001):340-348. |
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