中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Inhibition of hepatitis B virus by hammerhead ribozyme targeted to the poly(A) signal sequence in cultured cells

文献类型:期刊论文

作者Feng, Y; Kong, YY; Wang, Y; Qi, GR
刊名BIOLOGICAL CHEMISTRY
出版日期2001
卷号382期号:4页码:655-660
关键词antiviral hammerhead ribozyme HBV poly(A) signal viral replication
通讯作者Qi, GR (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要The deviant poly(A) signal of hepatitis B virus (HBV) not only controls the formation of the 3' end of all the viral RNA, but is also crucial for HBV replication. Hence, a cia-releasing hammerhead ribozyme (RzA) targeted to the poly(A) signal region of HBV subtype adr was investigated for its antiviral effects. In vitro, RzA cleaved HBV RNA at its target site up to 70%, while the disabled ribozyme (dRzA), which had a one-base mutation in the catalytic site, did not cleave the target RNA at all. When the ribozymes were cotransfected into HepG2 cells with the HBV genome-containing plasmid p3.611, the wild-type ribozyme RzA could effectively decrease HBV RNA levels and inhibit HBV replication, whereas its disabled form, dRzA, had much weaker effects, indicating that the active catalytic domain of the hammerhead ribozyme could markedly increase the extent of antisense-mediated inhibition. In addition, there was a gradient of effectiveness: the higher the amount of released ribozyme, the more the reduction in target HBV RNA in cells as well as progeny DNA reduction. These results suggest the possibility of the hammerhead ribozyme RzA to be used for the gene therapy of HBV infection.
学科主题Biochemistry & Molecular Biology
类目[WOS]Biochemistry & Molecular Biology
关键词[WOS]X GENE-EXPRESSION ; IN-VITRO ; ANTIVIRAL AGENTS ; HEPADNAVIRUS ; CLEAVAGE ; INVITRO
收录类别SCI
语种英语
WOS记录号WOS:000168970600014
版本出版稿
源URL[http://202.127.25.143/handle/331003/2655]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714
Feng, Y,Kong, YY,Wang, Y,et al. Inhibition of hepatitis B virus by hammerhead ribozyme targeted to the poly(A) signal sequence in cultured cells[J]. BIOLOGICAL CHEMISTRY,2001,382(4):655-660.
APA Feng, Y,Kong, YY,Wang, Y,&Qi, GR.(2001).Inhibition of hepatitis B virus by hammerhead ribozyme targeted to the poly(A) signal sequence in cultured cells.BIOLOGICAL CHEMISTRY,382(4),655-660.
MLA Feng, Y,et al."Inhibition of hepatitis B virus by hammerhead ribozyme targeted to the poly(A) signal sequence in cultured cells".BIOLOGICAL CHEMISTRY 382.4(2001):655-660.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。