Inhibition of hepatitis B virus by hammerhead ribozyme targeted to the poly(A) signal sequence in cultured cells
文献类型：期刊论文
| 作者 | Feng, Y; Kong, YY; Wang, Y; Qi, GR |
| 刊名 | BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2001 |
| 卷号 | 382期号:4页码:655-660 |
| 关键词 | antiviral hammerhead ribozyme HBV poly(A) signal viral replication |
| 通讯作者 | Qi, GR (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | The deviant poly(A) signal of hepatitis B virus (HBV) not only controls the formation of the 3' end of all the viral RNA, but is also crucial for HBV replication. Hence, a cia-releasing hammerhead ribozyme (RzA) targeted to the poly(A) signal region of HBV subtype adr was investigated for its antiviral effects. In vitro, RzA cleaved HBV RNA at its target site up to 70%, while the disabled ribozyme (dRzA), which had a one-base mutation in the catalytic site, did not cleave the target RNA at all. When the ribozymes were cotransfected into HepG2 cells with the HBV genome-containing plasmid p3.611, the wild-type ribozyme RzA could effectively decrease HBV RNA levels and inhibit HBV replication, whereas its disabled form, dRzA, had much weaker effects, indicating that the active catalytic domain of the hammerhead ribozyme could markedly increase the extent of antisense-mediated inhibition. In addition, there was a gradient of effectiveness: the higher the amount of released ribozyme, the more the reduction in target HBV RNA in cells as well as progeny DNA reduction. These results suggest the possibility of the hammerhead ribozyme RzA to be used for the gene therapy of HBV infection. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | X GENE-EXPRESSION ; IN-VITRO ; ANTIVIRAL AGENTS ; HEPADNAVIRUS ; CLEAVAGE ; INVITRO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000168970600014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2655]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Feng, Y,Kong, YY,Wang, Y,et al. Inhibition of hepatitis B virus by hammerhead ribozyme targeted to the poly(A) signal sequence in cultured cells[J]. BIOLOGICAL CHEMISTRY,2001,382(4):655-660. |
| APA | Feng, Y,Kong, YY,Wang, Y,&Qi, GR.(2001).Inhibition of hepatitis B virus by hammerhead ribozyme targeted to the poly(A) signal sequence in cultured cells.BIOLOGICAL CHEMISTRY,382(4),655-660. |
| MLA | Feng, Y,et al."Inhibition of hepatitis B virus by hammerhead ribozyme targeted to the poly(A) signal sequence in cultured cells".BIOLOGICAL CHEMISTRY 382.4(2001):655-660. |
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