FUP1, a gene associated with hepatocellular carcinoma, stimulates NIH3T3 cell proliferation and tumor formation in nude mice
文献类型：期刊论文
| 作者 | Pan, W; Zhang, Q; Xi, QS; Gan, RB; Li, TP |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2001 |
| 卷号 | 286期号:5页码:1033-1038 |
| 关键词 | hepatocellular carcinoma NIH3T3 BEL7404 stable transfection antisense tumorigenicity |
| 通讯作者 | Li, TP (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, POB 55,320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Human primary hepatocellular carcinoma (HCC) is one of the highly prevalent malignant diseases worldwide, the identification of HCC-associated genes has been a major approach in elucidating the molecular mechanism of tumorigenesis of HCC. In our previous studies, a function-unknown gene, which displayed marked expression difference between the HCC sample and normal liver control has been detected by cDNA microarray. This gene was named after fup1 (function-unknown protein 1), and was cloned according to the data of GenBank. The cDNA of fup1 has an open-reading frame 1233 base pairs in size. Here, the function analysis of FUP1 related to HCC is being reported. The NIH3T3 cells transiently transfected with FLAG-conjugated FUP1 revealed strong nuclear staining in immunofluorescent assay. Furthermore, cell proliferation enhancing activity of fup1 was shown by MTT assay in stable transfectant NIH3T3 cell line with pcDNA3-derived plasmid having fup1 under the regulation of pCMV, while cell proliferation repressing activity of antisense fup1 was observed in BEL7404 stable transfectant cells. Tumorigenicity of the above stable transfectant cells was analyzed in nude mice compared with appropriate controls. The result was in good agreement with MTT assay. Elevated tumorigenicity of fup1 transfected NIH3T3 cell and repressed tumorigenicity of antisense fup1 transfected BEL7404 cell were clearly demonstrated. The results above suggested that fup1 might be a critical gene related to carcinogenesis of HCC. Detailed molecular function of fup1 remains to be elucidated. (C) 2001 Academic Press. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | EXPRESSION ; MUTATIONS ; INHIBITION ; SEQUENCE ; GROWTH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000170966200031 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2664]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Pan, W,Zhang, Q,Xi, QS,et al. FUP1, a gene associated with hepatocellular carcinoma, stimulates NIH3T3 cell proliferation and tumor formation in nude mice[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2001,286(5):1033-1038. |
| APA | Pan, W,Zhang, Q,Xi, QS,Gan, RB,&Li, TP.(2001).FUP1, a gene associated with hepatocellular carcinoma, stimulates NIH3T3 cell proliferation and tumor formation in nude mice.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,286(5),1033-1038. |
| MLA | Pan, W,et al."FUP1, a gene associated with hepatocellular carcinoma, stimulates NIH3T3 cell proliferation and tumor formation in nude mice".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 286.5(2001):1033-1038. |
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