High frequency of homologous recombination in the genome of modified vaccinia virus Ankara strain (MVA)
文献类型：期刊论文
| 作者 | Zhu, LX; Xie, YH; Li, GD; Wang, Y |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 33期号:5页码:497-503 |
| 关键词 | recombination frequency homologous recombination vaccinia virus MVA |
| 通讯作者 | , |
| 英文摘要 | MVA is a genetically modified vaccinia virus strain, which is replication defective and extremely safe for research and clinical use. To further improve the safety of MVA and achieve more efficient selection of recombinant constructs, a transient selection marker system has been developed, which contains vaccinia virus k1l gene flanked by two identical fragments. In this report, four recombinant MVAs were constructed with this system, and homologous recombination of recombinant MVAs was studied during the construction procedure. The results showed that the recombination frequency of these rMVAs was significantly high, though tower than that observed in other vaccinia virus strains. The k1l free rMVAs could be conveniently obtained after 3 or 4 blind passages. Our data indicated that recombinant MVA with a transient selection marker system was safe for use as live vaccine and gene therapy vector for human. In addition, blind passages could enhance the efficiency in isolation of k1l free recombinants. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | T-LYMPHOCYTE RESPONSE ; ENVELOPE PROTEIN ; IMMUNIZATION ; HOST ; INDUCTION ; CHALLENGE ; DELIVERY ; PRIMES ; CELLS ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000171403600005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2666]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhu, LX,Xie, YH,Li, GD,et al. High frequency of homologous recombination in the genome of modified vaccinia virus Ankara strain (MVA)[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(5):497-503. |
| APA | Zhu, LX,Xie, YH,Li, GD,&Wang, Y.(2001).High frequency of homologous recombination in the genome of modified vaccinia virus Ankara strain (MVA).ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(5),497-503. |
| MLA | Zhu, LX,et al."High frequency of homologous recombination in the genome of modified vaccinia virus Ankara strain (MVA)".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.5(2001):497-503. |
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