Screening an alpha-glucosidase inhibitor from a phage-displayed peptide library
文献类型：期刊论文
| 作者 | Li, JD; Wang, KY |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 33期号:5页码:513-518 |
| 关键词 | phage-display peptide library biopanning alpha-glucosidase inhibitor |
| 通讯作者 | , |
| 英文摘要 | A phage-displayed peptide library was biopanned with Aspergillus niger glucoamylase. Three specifically binding phage clones (GB-1, GB-2 and GB-3) were identified after three cycles of screening and amplification All three peptides contained a disulfide bridge, and the impairment of disulfide bond greatly lowered the binding activity. One peptide, cyclic KCHFEECLAY, representing the N-terminal 10 amino acid residues from GB-1, competitively inhibited A. niger glucoamylase (K-i = 0. 2 mmol/L) as well as rat intestinal alpha -glucosidase (K-i = 1. 4 mmol/L). |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | EPITOPE LIBRARY ; BINDING ; IDENTIFICATION ; GLYCOSYLATION ; ANTIBODY ; LIGANDS |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000171403600007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2668]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, JD,Wang, KY. Screening an alpha-glucosidase inhibitor from a phage-displayed peptide library[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(5):513-518. |
| APA | Li, JD,&Wang, KY.(2001).Screening an alpha-glucosidase inhibitor from a phage-displayed peptide library.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(5),513-518. |
| MLA | Li, JD,et al."Screening an alpha-glucosidase inhibitor from a phage-displayed peptide library".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.5(2001):513-518. |
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