Functional analysis of helicase gene promoter and homologous region 3 enhancer in Bombyx mori nuclear polyhedrosis virus
文献类型：期刊论文
| 作者 | Xiao, QL; Zhang, ZF; Yi, YZ; He, JL; Wu, XF |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 33期号:5页码:525-530 |
| 关键词 | Bombyx mori nuclear polyhedrosis virus helicase gene promoter enhancer transient expression |
| 通讯作者 | , |
| 英文摘要 | The promoter of the helicase gene, including 510 bp upstream of ATG, was cloned and sequenced, and was found that it had both early and late RNA initiation sites. The initiation codon ATG was deleted by using point mutation. Luciferase gene, as a reporter gene, was fused with the promoter region to construct the plsmid pBmhel510luc. When pBmhel510luc was transfected into Bm-5 and Sf-21 cell lines, the helicase gene promoter was recognized by cellular RNA polymerase and transactivated by viral factors. Baculovirus homologous regions (hrs) act as viral DNA replication start sites, which also have been shown to alter the rate of transcription for cis-linked promoters. BmNPV hr3 was cloned into a downstream site of luc gene, to study the effect of this enhancer on hel510 promoter activity. The transient expression in transfected insect cell lines and silkworm larvae indicated that hr3 could enhance the transcriptional level of hel510 promoter by about 7 000 and 1 000 fold, respectively. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | NUCLEOTIDE-SEQUENCE ; P143 GENE ; DNA-SEQUENCE ; EXPRESSION ; TRANSCRIPTION ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000171403600009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2669]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xiao, QL,Zhang, ZF,Yi, YZ,et al. Functional analysis of helicase gene promoter and homologous region 3 enhancer in Bombyx mori nuclear polyhedrosis virus[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(5):525-530. |
| APA | Xiao, QL,Zhang, ZF,Yi, YZ,He, JL,&Wu, XF.(2001).Functional analysis of helicase gene promoter and homologous region 3 enhancer in Bombyx mori nuclear polyhedrosis virus.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(5),525-530. |
| MLA | Xiao, QL,et al."Functional analysis of helicase gene promoter and homologous region 3 enhancer in Bombyx mori nuclear polyhedrosis virus".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.5(2001):525-530. |
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