中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Functional analysis of helicase gene promoter and homologous region 3 enhancer in Bombyx mori nuclear polyhedrosis virus

文献类型:期刊论文

作者Xiao, QL; Zhang, ZF; Yi, YZ; He, JL; Wu, XF
刊名ACTA BIOCHIMICA ET BIOPHYSICA SINICA
出版日期2001
卷号33期号:5页码:525-530
关键词Bombyx mori nuclear polyhedrosis virus helicase gene promoter enhancer transient expression
通讯作者,
英文摘要The promoter of the helicase gene, including 510 bp upstream of ATG, was cloned and sequenced, and was found that it had both early and late RNA initiation sites. The initiation codon ATG was deleted by using point mutation. Luciferase gene, as a reporter gene, was fused with the promoter region to construct the plsmid pBmhel510luc. When pBmhel510luc was transfected into Bm-5 and Sf-21 cell lines, the helicase gene promoter was recognized by cellular RNA polymerase and transactivated by viral factors. Baculovirus homologous regions (hrs) act as viral DNA replication start sites, which also have been shown to alter the rate of transcription for cis-linked promoters. BmNPV hr3 was cloned into a downstream site of luc gene, to study the effect of this enhancer on hel510 promoter activity. The transient expression in transfected insect cell lines and silkworm larvae indicated that hr3 could enhance the transcriptional level of hel510 promoter by about 7 000 and 1 000 fold, respectively.
学科主题Biochemistry & Molecular Biology; Biophysics
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]NUCLEOTIDE-SEQUENCE ; P143 GENE ; DNA-SEQUENCE ; EXPRESSION ; TRANSCRIPTION ; PROTEIN
收录类别SCI
语种中文
WOS记录号WOS:000171403600009
版本出版稿
源URL[http://202.127.25.143/handle/331003/2669]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
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GB/T 7714
Xiao, QL,Zhang, ZF,Yi, YZ,et al. Functional analysis of helicase gene promoter and homologous region 3 enhancer in Bombyx mori nuclear polyhedrosis virus[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(5):525-530.
APA Xiao, QL,Zhang, ZF,Yi, YZ,He, JL,&Wu, XF.(2001).Functional analysis of helicase gene promoter and homologous region 3 enhancer in Bombyx mori nuclear polyhedrosis virus.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(5),525-530.
MLA Xiao, QL,et al."Functional analysis of helicase gene promoter and homologous region 3 enhancer in Bombyx mori nuclear polyhedrosis virus".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.5(2001):525-530.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

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