Cloning and distribution of rRP58, a novel neuronal gene
文献类型:期刊论文
作者 | Kang, JS; Yang, YC; Liu, HL; Li, YH; Du, YC; Li, RX |
刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
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出版日期 | 2001 |
卷号 | 33期号:5页码:563-568 |
关键词 | rRP58 zinc finger domain POZ rat brain antibody |
通讯作者 | , |
英文摘要 | EST (AW055733) is a 3'-cDNA fragment specially expressed in rat brain. It is homologous to human and mouse RP58 gene, which is a transcriptional repressor gene. Primers were designed based on these two genes, and then two transcripts of rRP58 gene were got from male SD rat using RT-PCR method. The rRP58 protein is a C2H2 type zinc finger protein, containing POZ domain at N-terminal and zinc finger domain (ZFD) at C-terminal. In addition, there is a highly acidic region between POZ and ZFD. POZ and ZFD were cloned, expressed and purified. Then, the antibodies to POZ and ZFD were prepared, and used to analyze distribution of alternative transcripts of rRP58. |
学科主题 | Biochemistry & Molecular Biology; Biophysics |
类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
关键词[WOS] | PROTEIN INTERACTION MOTIF ; DOMAIN ; ACTIVATION ; REGION ; RP58 |
收录类别 | SCI |
语种 | 中文 |
WOS记录号 | WOS:000171403600016 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/2670] ![]() |
专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
推荐引用方式 GB/T 7714 | Kang, JS,Yang, YC,Liu, HL,et al. Cloning and distribution of rRP58, a novel neuronal gene[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(5):563-568. |
APA | Kang, JS,Yang, YC,Liu, HL,Li, YH,Du, YC,&Li, RX.(2001).Cloning and distribution of rRP58, a novel neuronal gene.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(5),563-568. |
MLA | Kang, JS,et al."Cloning and distribution of rRP58, a novel neuronal gene".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.5(2001):563-568. |
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