Regulation of CDC42 GTPase by proline-rich tyrosine kinase 2 interacting with PSGAP, a novel pleckstrin homology and Src homology 3 domain containing rhoGAP protein
文献类型：期刊论文
| 作者 | Ren, XR; Du, QS; Huang, YZ; Ao, SZ; Mei, L; Xiong, WC |
| 刊名 | JOURNAL OF CELL BIOLOGY
 |
| 出版日期 | 2001 |
| 卷号 | 152期号:5页码:971-983 |
| 关键词 | proline-rich tyrosine kinase 2 focal adhesion kinase CDC42 rhoGAP PSGAP |
| 通讯作者 | Xiong, WC (reprint author), Univ Alabama, Dept Pathol, Birmingham, AL 35294 USA., |
| 英文摘要 | Proline-rich tyrosine kinase 2 (PYK2), a tyrosine kinase structurally related to focal adhesion kinase (FAK), is implicated in regulating cytoskeletal organization. However, mechanisms by which PYK2 participates in and regulates cytoskeletal organization remain largely unknown. Here we report identification of PSGAP, a novel protein that interacts with PYK2 and FAK and contains multiple domains including a pleckstrin homology domain, a rhoGTPase-activating protein domain, and a Src homology 3 domain. PYK2 interacts with PSGAP Src homology 3 domain via the carboxyl-terminal proline-rich sequence. PSGAP is able to increase GTPase activity of CDC42 and RhoA in vitro and in vivo. Remarkably, PYK2, but not FAK, can activate CDC42 via inhibition of PSGAP-mediated GTP hydrolysis of CDC42. Moreover, PSGAP is Localized at cell periphery in fibroblasts in a pleckstrin homology domain-dependent manner. Over expression of PSGAP in fibroblasts results in reorganization of cytoskeletal structures and changes of cellular morphology, which requires rhoGTPase-activating activity. Taken together, our results suggest that PSGAP is a signaling protein essential for PYK2 regulation of cytoskeletal organization via Rho family GTPases. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | FOCAL ADHESION KINASE ; ACTIN STRESS FIBERS ; CELL-ADHESION ; ACTIVATING-PROTEIN ; SH3 DOMAINS ; EXTRACELLULAR-MATRIX ; SIGNAL-TRANSDUCTION ; B-CELLS ; V-CRK ; IDENTIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000167408000012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2672]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ren, XR,Du, QS,Huang, YZ,et al. Regulation of CDC42 GTPase by proline-rich tyrosine kinase 2 interacting with PSGAP, a novel pleckstrin homology and Src homology 3 domain containing rhoGAP protein[J]. JOURNAL OF CELL BIOLOGY,2001,152(5):971-983. |
| APA | Ren, XR,Du, QS,Huang, YZ,Ao, SZ,Mei, L,&Xiong, WC.(2001).Regulation of CDC42 GTPase by proline-rich tyrosine kinase 2 interacting with PSGAP, a novel pleckstrin homology and Src homology 3 domain containing rhoGAP protein.JOURNAL OF CELL BIOLOGY,152(5),971-983. |
| MLA | Ren, XR,et al."Regulation of CDC42 GTPase by proline-rich tyrosine kinase 2 interacting with PSGAP, a novel pleckstrin homology and Src homology 3 domain containing rhoGAP protein".JOURNAL OF CELL BIOLOGY 152.5(2001):971-983. |
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