Colony color assay coupled with 5FOA negative selection greatly improves yeast three-hybrid library screening efficiency
文献类型：期刊论文
| 作者 | He, YZ; Bao, L; Liu, DG |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2001 |
| 卷号 | 46期号:5页码:403-406 |
| 关键词 | colony color assay 5FOA negative selection yeast three-hybrid system RNA binding protein |
| 通讯作者 | Liu, DG (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | The recently developed yeast three-hybrid system is a powerful tool for analyzing RNA-protein interactions in vivo. However, large numbers of false positives are frequently met due to bait RNA-independent activation of the reporter gene in the library screening using this system. In this report, we coupled the colony color assay with the 5FOA (5-fluoroorotic acid) negative selection in the library screening, and found that this coupled method effectively eliminated bait RNA-independent false positives and hence greatly improved library screening efficiency. We used this method successfully in isolation of cDNA of an RNA-binding protein that might play important roles in certain cellular process. This improvement will facilitate the use of the yeast three-hybrid system in analyzing RNA-protein interaction. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | RNA-BINDING PROTEIN ; 3-HYBRID SYSTEM ; ELEGANS ; CELLS ; ACID |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000168244600014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2673]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | He, YZ,Bao, L,Liu, DG. Colony color assay coupled with 5FOA negative selection greatly improves yeast three-hybrid library screening efficiency[J]. CHINESE SCIENCE BULLETIN,2001,46(5):403-406. |
| APA | He, YZ,Bao, L,&Liu, DG.(2001).Colony color assay coupled with 5FOA negative selection greatly improves yeast three-hybrid library screening efficiency.CHINESE SCIENCE BULLETIN,46(5),403-406. |
| MLA | He, YZ,et al."Colony color assay coupled with 5FOA negative selection greatly improves yeast three-hybrid library screening efficiency".CHINESE SCIENCE BULLETIN 46.5(2001):403-406. |
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