Cloning and expression of targeting Mn-SOD to central nervous system in E-coli
文献类型：期刊论文
| 作者 | Zhang, YH; He, HJ; Yuan, QS; Yang, WD; Wu, XF |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2001 |
| 卷号 | 28期号:6页码:895-899 |
| 关键词 | manganese superoxide dismutase (Mn-SOD) tetanus toxin fragment C fusion expression central nervous system targeting dilivery |
| 通讯作者 | , |
| 英文摘要 | The nontoxic fragment C of tetanus toxin (TTC) can transport other proteins from the circulation to central nervous system motor neurons. Increased levels of CuZn-SOD are protective in experimental models of stroke and Parkinsons's diseases, where mutations in SOD may cause motor neuron disease. Here the human Mn-SOD is linked to tetanus toxin fragment C gene to construct the fusion gene, then was ligated into prokaryotic expression vector pET-22b (+) expression of the plasmid in E. coli, resulted in the production of a protein has a subunit molecular mass of 71 ku and is recognized by both anti-Mn-SOD and anti-tetanus toxin antibody. The Mn-SOD moiety retains substantial enzymatic activity, where the TTC moiety can delivers the fusion protein to central nervous system neurons. Such fusions should provide a powerful tool for investigating the protective and destructive roles of Mn-SOD in motor neurons. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | AMYOTROPHIC-LATERAL-SCLEROSIS ; SUPEROXIDE-DISMUTASE ; GENE |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000172947500029 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2676]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, YH,He, HJ,Yuan, QS,et al. Cloning and expression of targeting Mn-SOD to central nervous system in E-coli[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2001,28(6):895-899. |
| APA | Zhang, YH,He, HJ,Yuan, QS,Yang, WD,&Wu, XF.(2001).Cloning and expression of targeting Mn-SOD to central nervous system in E-coli.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,28(6),895-899. |
| MLA | Zhang, YH,et al."Cloning and expression of targeting Mn-SOD to central nervous system in E-coli".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 28.6(2001):895-899. |
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