cDNA cloning and expression of acutin II, a native mutant of thrombin-like enzyme from Agkistrodon acutus
文献类型：期刊论文
| 作者 | Pan, H; Liu, JW; Du, XY; Zhou, YC; Wu, XF |
| 刊名 | PROTEIN AND PEPTIDE LETTERS
 |
| 出版日期 | 2001 |
| 卷号 | 8期号:6页码:469-475 |
| 关键词 | Agkistrodon acutus snake venom thrombin-like enzyme RT-PCR acutinII cDNA cloning expression E. coli |
| 通讯作者 | , |
| 英文摘要 | One cDNA of a thrombin-like enzyme named acutinII was amplified by RT-PCR from the venom gland of the Agkistrodon acutus snake. AcutinII shows high similarity to acutin, the other thrombin-like enzyme from Agkistrodon acutus snake in nucleotide and amino acid sequences except it is shorter by 16 amino acid at the C-terminus. The catalytic residues and disulfide bridges of acutinII were deduced. AcutinII was expressed in E. coli. The expressed protein was detected by western blotting and the yield was up to 32% of the total proteins. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | SNAKE-VENOM ENZYME ; MOLECULAR-CLONING ; SEQUENCE-ANALYSIS ; HALYS PALLAS ; BATROXOBIN ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000172208500007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2677]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Pan, H,Liu, JW,Du, XY,et al. cDNA cloning and expression of acutin II, a native mutant of thrombin-like enzyme from Agkistrodon acutus[J]. PROTEIN AND PEPTIDE LETTERS,2001,8(6):469-475. |
| APA | Pan, H,Liu, JW,Du, XY,Zhou, YC,&Wu, XF.(2001).cDNA cloning and expression of acutin II, a native mutant of thrombin-like enzyme from Agkistrodon acutus.PROTEIN AND PEPTIDE LETTERS,8(6),469-475. |
| MLA | Pan, H,et al."cDNA cloning and expression of acutin II, a native mutant of thrombin-like enzyme from Agkistrodon acutus".PROTEIN AND PEPTIDE LETTERS 8.6(2001):469-475. |
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