Study of rat neuronal genes with ordered differential display method
文献类型：期刊论文
| 作者 | Kang, JS; Wang, ZP; Jin, ML; Guo, N; Wang, T; Du, YC; Li, RX |
| 刊名 | SCIENCE IN CHINA SERIES C-LIFE SCIENCES
 |
| 出版日期 | 2001 |
| 卷号 | 44期号:6页码:601-609 |
| 关键词 | ODD rat brain EST antisense oligodeoxynucleotides ethology |
| 通讯作者 | Li, RX (reprint author), Chinese Acad Sci, Inst Biol Sci, Shanghai Res Ctr Biotechnol, Shanghai 200233, Peoples R China., |
| 英文摘要 | Ordered differential display (ODD) was developed recently and has been applied to systematic comparison of expression profiles of genes. It was further improved with the specific complexing property between biotin and streptavidin by the authors. First, random primer and biotinylated oligo (dT) primer were used to make pools of double strand cDNA. Second, streptavidin-coated PCR tube is used to absorb 3'ESTs specifically to avoid the negative effect of other DNA fragments. In the case of 3'ESTs comparison patterns between embryonic brain and body of SD rat, more than forty differentially expressed genes were cloned and identified. The function of rZIC gene, one of the genes identified and cloned, was studied through ethological experiments. The result showed that rZIC gene was associated with locomotion activity of adult mice. |
| 学科主题 | Life Sciences & Biomedicine - Other Topics |
| 类目[WOS] | Biology |
| 关键词[WOS] | EXPRESSION PROFILES ; MOLECULAR-CLONING ; MESSENGER-RNA ; CDNA ; PROTEIN ; SEQUENCE ; CELLS ; DNA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000175029600006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2680]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Kang, JS,Wang, ZP,Jin, ML,et al. Study of rat neuronal genes with ordered differential display method[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2001,44(6):601-609. |
| APA | Kang, JS.,Wang, ZP.,Jin, ML.,Guo, N.,Wang, T.,...&Li, RX.(2001).Study of rat neuronal genes with ordered differential display method.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,44(6),601-609. |
| MLA | Kang, JS,et al."Study of rat neuronal genes with ordered differential display method".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 44.6(2001):601-609. |
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