PCR based cloning and sequence analysis of the Pichia pastoris cystathionine beta-Synthase gene
文献类型：期刊论文
| 作者 | Li, DY; Ji, XS; Yu, J; Chen, MJ; Yuan, ZY |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 33期号:6页码:600-606 |
| 关键词 | cystathionine beta-synthase Pichia pastoris RACE cloning alignment |
| 通讯作者 | , |
| 英文摘要 | The cystathionine P-Synthase (CBS) gene of Pichia pastoris has been cloned by homology to the CBS gene of Saccharomyes cerevisiae. First, based on the homology alignment of CBS genes from different sources, a pair of degenerate PCR primers were designed to amplify a conservative fragment of Pichia pastoris CBS gene. After the sequence was revealed, 5' RACE and 3' RACE were performed separately. The whole sequence of Pichia pasotris CBS gene was assembled. This gene encodes a polypeptide of 501 residues. Comparison of deduced amino acid sequence of this new gene with that of S. cerevisiae CBS showed 54 % identity. Disruption of CBS gene resulted in a Cys (-) auxotrophy in Pichia pastoris. The sequence was submitted to GenBank/EMBL/DDBJ under Accession No. AF367364. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | SACCHAROMYCES-CEREVISIAE ; YEAST ; EXPRESSION ; SYSTEM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000172362600002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2684]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, DY,Ji, XS,Yu, J,et al. PCR based cloning and sequence analysis of the Pichia pastoris cystathionine beta-Synthase gene[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(6):600-606. |
| APA | Li, DY,Ji, XS,Yu, J,Chen, MJ,&Yuan, ZY.(2001).PCR based cloning and sequence analysis of the Pichia pastoris cystathionine beta-Synthase gene.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(6),600-606. |
| MLA | Li, DY,et al."PCR based cloning and sequence analysis of the Pichia pastoris cystathionine beta-Synthase gene".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.6(2001):600-606. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。