Effect of downstream sequence on the cleavage of envelop protein 1 signal sequence in hepatitis C virus
文献类型：期刊论文
| 作者 | Zhu, LX; Kong, YY; Wang, Y; Li, GD |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 33期号:6页码:682-686 |
| 关键词 | hepatitis C virus (HCV) E1 signal sequence post-translational processing |
| 通讯作者 | , |
| 英文摘要 | The RNA genome of hepatitis C virus encodes a polyprotein of similar to 3000 amino acids, which is processed into 10 viral proteins by proteases provided by host cells and virus itself. Multiple precursors are produced due to inefficient processing. Here, the study of E1 signal sequence (C/E1 site) processing in eukaryotic vaccinia virus/T7 system is reported. Differently truncated HCV structural proteins were expressed in this system. It was found that the efficient cleavage of E1 signal sequence was affected by downstream envelop protein sequences. When the lacZ gene encoding a product with similar size was engineered downstream to the E1 signal sequence, the inefficient cleavage of signal sequence was also observed, suggesting that the effect of downstream sequence on the cleavage was due to the presence of the envelop protein sequences. Computer-aided analysis clearly showed that E1 signal sequences was a typical signal sequence. The influence of downstream sequences to signal sequence cleavage demonstrated here was uncommon. To date, similar observations were only reported for the processing of IL-12 signal sequence and the C/prM site of flavivirus. As both flavivirus and HCV are classified into the same Flaviviridae family, this downstream-sequence-related cleavage of signal sequence worths further studying. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ENDOPLASMIC-RETICULUM ; EXPRESSION ; IDENTIFICATION ; PEPTIDE ; REGION ; CELLS ; E1 |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000172362600015 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2689]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhu, LX,Kong, YY,Wang, Y,et al. Effect of downstream sequence on the cleavage of envelop protein 1 signal sequence in hepatitis C virus[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(6):682-686. |
| APA | Zhu, LX,Kong, YY,Wang, Y,&Li, GD.(2001).Effect of downstream sequence on the cleavage of envelop protein 1 signal sequence in hepatitis C virus.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(6),682-686. |
| MLA | Zhu, LX,et al."Effect of downstream sequence on the cleavage of envelop protein 1 signal sequence in hepatitis C virus".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.6(2001):682-686. |
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