Fluorescent staining of glycoproteins on polyvinylidene difluoride membrane with 8-aminonaphthalene-1,3,6-trisulfonate
文献类型：期刊论文
| 作者 | Che, FY; Song, JF; Yu, LR; Wang, KY; Xia, QC |
| 刊名 | BIOTECHNIQUES
 |
| 出版日期 | 2001 |
| 卷号 | 30期号:6页码:1272-1272 |
| 通讯作者 | Xia, QC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Here, we describe a simple and sensitive method that allows fluorescent detection of glycoproteins on polyvinylidene difluoride (PVDF) membrane. We used periodic acid oxidation of carbohydrate chains of glycoproteins and fluorescent labeling with 8-aminoaphthalene-1, 3,6-trisulfonate (ANTS) by reductive amination. We developed an additional method to enhance the ability of PVDF to absorb glycoproteins by using non-glycoprotein lectin, such as wheat germ agglutinin (WGA), as a link between the PVDF membrane and glycoproteins, resulting in considerably increased detection sensitivity to glycoproteins. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| 关键词[WOS] | DIGOXIGENIN-LABELED LECTINS ; CAPILLARY-ELECTROPHORESIS ; POLYACRYLAMIDE GELS ; OLIGOSACCHARIDES ; SEPARATION ; ACID ; CHEMILUMINESCENCE ; TECHNOLOGY ; COMPLEX ; BIOTIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000169252200016 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2692]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Che, FY,Song, JF,Yu, LR,et al. Fluorescent staining of glycoproteins on polyvinylidene difluoride membrane with 8-aminonaphthalene-1,3,6-trisulfonate[J]. BIOTECHNIQUES,2001,30(6):1272-1272. |
| APA | Che, FY,Song, JF,Yu, LR,Wang, KY,&Xia, QC.(2001).Fluorescent staining of glycoproteins on polyvinylidene difluoride membrane with 8-aminonaphthalene-1,3,6-trisulfonate.BIOTECHNIQUES,30(6),1272-1272. |
| MLA | Che, FY,et al."Fluorescent staining of glycoproteins on polyvinylidene difluoride membrane with 8-aminonaphthalene-1,3,6-trisulfonate".BIOTECHNIQUES 30.6(2001):1272-1272. |
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