Toward understanding the role of insulin alpha-helix II in the growth-promoting activity of insulin
文献类型：期刊论文
| 作者 | Chen, H; Feng, YM |
| 刊名 | BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2001 |
| 卷号 | 382期号:7页码:1057-1062 |
| 关键词 | growth-promoting activity insulin insulin-like growth factor 1 (IGF-1) molecular scaffold structural motif |
| 通讯作者 | Feng, YM (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Tang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | For further understanding the contribution of the alpha -helix II (alpha II) in the growth-promoting activity of insulin, the residues A2IIe, A5Gln, and A8Thr located in alpha II were mutated to Leu, Glu, and Tyr, respectively. Three mutant insulins, [A2Leu]human insulin, [A5Glu]human insulin, and [A8Tyr]human insulin, were prepared by means of site-directed mutagenesis. The in vitro growth-promoting activities of the three mutant insulins, measured using GR2H6 cells, were 7.5%, 291%, and 250% of that of native insulin, respectively. Their receptor-binding activities to the insulin receptor were 2.3%, 46.7%, and 138.7%, respectively, compared with native insulin. Both the growth-promoting and receptor-binding activities of [A2Leu]human insulin and [A3Leu]insulin (Shi et al., 1997) were parallel and greatly decreased compared with native insulin. The results demonstrate that the residues A2IIe and A3Val in the all are essential for the growth-promoting activity of insulin, and the growth-promoting function of insulin might be performed through, or mainly through, binding to the insulin receptor. The growth-promoting activities of [A5Glu]human insulin and [A8Tyr]human insulin were increased 6-fold and 2-fold, respectively, compared with native insulin, indicating that their growth-promoting activities might be expressed by, or mainly by, binding to the IGF-1 receptor. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | RECEPTOR-BINDING ; HYBRID ; AMPHIOXUS ; CHAIN ; EVOLUTION ; PEPTIDE ; 1.5-A ; IGF-1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000170535100008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2694]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, H,Feng, YM. Toward understanding the role of insulin alpha-helix II in the growth-promoting activity of insulin[J]. BIOLOGICAL CHEMISTRY,2001,382(7):1057-1062. |
| APA | Chen, H,&Feng, YM.(2001).Toward understanding the role of insulin alpha-helix II in the growth-promoting activity of insulin.BIOLOGICAL CHEMISTRY,382(7),1057-1062. |
| MLA | Chen, H,et al."Toward understanding the role of insulin alpha-helix II in the growth-promoting activity of insulin".BIOLOGICAL CHEMISTRY 382.7(2001):1057-1062. |
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