Roles of IL-4 and other factors in trichosanthin-induced ovalbumin-specific IgE response
文献类型：期刊论文
| 作者 | Xu, WF; Ji, YY; Wu, YD; Lin, GM; Ye, M; Sun, B |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 22期号:8页码:736-740 |
| 关键词 | trichosanthin immunoglobulins interleukin-4 interleukin-13 tumor necrosis factor ligands |
| 通讯作者 | Sun, B (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Mol Immunol, Shanghai 200031, Peoples R China., |
| 英文摘要 | AIM: To study the mechanism of trichosanthin (TCS)induced ovalbumin (OVA)-specific immunoglobulin E (IgE) response in vivo. METHODS: To determine whether interleukin-4 ( IL-4) was involved in TCS-induced IgE production, TCS and OVA co-immunized mice were treated with anti-IL-4 monoclonal antibodies (mAb) and OVA-specific serum IgE production was measured by enzyme-linked immunosorbent assay (ELISA). To distinguish whether recombinant IL-4 (rIL-4) was sufficient to support OVA-specific IgE response, OVA alone immunized mice were treated with rIL-4 and OVA-induced IgE production were examined by ELISA in the serum. To determine whether additional factors were involved in TCS-induced IgE response, the kinetic expression of CD40 ligand ( CD40L), tumor necrosis factor-alpha (TNF-alpha), and interleukin-13 (IL-13) were measured by semi-quantitative RT-PCR in draining mesenteric lymph node (MLN) from TCS-immunized mice. RESULTS: TCS-induced OVA-specific IgE production was suppressed by anti-IL-4 antibody, whereas IL-4 alone could not induce OVA-specific IgE production. CD40L, TNF-alpha, and IL-13 all expressed high levels in MLN after both primary and secondary immune responses. Among them CD40L had the similar transient expression peak to that of IL-4. CONCLUSION: IL-4 was indispensable for TCS-induced OVA-specific IgE production, and the other three factors examined may also be involved in, but CD40L may play a more important role. |
| 学科主题 | Chemistry; Pharmacology & Pharmacy |
| 类目[WOS] | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 关键词[WOS] | NECROSIS-FACTOR-ALPHA ; HUMAN B-CELLS ; INFLAMMATORY RESPONSES ; COSTIMULATORY SIGNAL ; TNF-ALPHA ; T-CELLS ; ACTIVATION ; INDUCTION ; COMPLEX ; GLQ223 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000170414900014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2698]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xu, WF,Ji, YY,Wu, YD,et al. Roles of IL-4 and other factors in trichosanthin-induced ovalbumin-specific IgE response[J]. ACTA PHARMACOLOGICA SINICA,2001,22(8):736-740. |
| APA | Xu, WF,Ji, YY,Wu, YD,Lin, GM,Ye, M,&Sun, B.(2001).Roles of IL-4 and other factors in trichosanthin-induced ovalbumin-specific IgE response.ACTA PHARMACOLOGICA SINICA,22(8),736-740. |
| MLA | Xu, WF,et al."Roles of IL-4 and other factors in trichosanthin-induced ovalbumin-specific IgE response".ACTA PHARMACOLOGICA SINICA 22.8(2001):736-740. |
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