Characterization of the separate kinase domain of chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
文献类型：期刊论文
| 作者 | Yang, QH; Zhu, Z; Li, L |
| 刊名 | PROGRESS IN NATURAL SCIENCE
 |
| 出版日期 | 2001 |
| 卷号 | 11期号:8页码:578-586 |
| 关键词 | 6-phosphofructo-2-kinase fructose-2 glutathione S-transferase fusion protein kinetic property 6-bisphosphatase |
| 通讯作者 | Li, L (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | The bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase consists of two distinct domains which catalyze the synthesis and hydrolysis of fructose-2, 6-bisphosphate, respectively. In this work the properties of the separate 6-phosphofructo-2-kinase domain were investigated. Purification of the expressed separate domain or isolation of this domain from purified glutathione S-transferase (GST) fusion protein with thrombin cleavage led to the loss of its kinase activity. Thus the domain in the GST-tagged form was characterized, The two forms of the domain with different lengths (amino acids 1 similar to 249 and 1 similar to 286) were very similar in kinetic property and could catalyze the formation of fructose-2,6-bisphosphate with a k(cat) 4-fold lower than that of the full-length enzyme. In addition, the domain was much more sensitive to guanidine inactivation and heat denaturation, and less stable at pH values below 7 than the full-length enzyme. The results suggest that the separate kinase domain of the bifunctional enzyme is far less perfect in structure in the absence of the bisphosphatase domain, though it still possesses the 6-phosphofructo-2-kinase activity. |
| 学科主题 | Materials Science; Science & Technology - Other Topics |
| 类目[WOS] | Materials Science, Multidisciplinary ; Multidisciplinary Sciences |
| 关键词[WOS] | SITE-DIRECTED MUTAGENESIS ; RAT 6-PHOSPHOFRUCTO-2-KINASE FRUCTOSE-2,6-BISPHOSPHATASE ; ESCHERICHIA-COLI ; BIFUNCTIONAL ENZYME ; BISPHOSPHATASE DOMAIN ; CRYSTAL-STRUCTURE ; 2-KINASE DOMAIN ; BINDING-SITE ; EXPRESSION ; TERMINUS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000170436000003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2699]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yang, QH,Zhu, Z,Li, L. Characterization of the separate kinase domain of chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase[J]. PROGRESS IN NATURAL SCIENCE,2001,11(8):578-586. |
| APA | Yang, QH,Zhu, Z,&Li, L.(2001).Characterization of the separate kinase domain of chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.PROGRESS IN NATURAL SCIENCE,11(8),578-586. |
| MLA | Yang, QH,et al."Characterization of the separate kinase domain of chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase".PROGRESS IN NATURAL SCIENCE 11.8(2001):578-586. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。