Non-radioisotopic method for the in vitro measurement of EGF receptor tyrosine kinase
文献类型：期刊论文
| 作者 | Ge, GX; Wu, J; Lin, QS |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2001 |
| 卷号 | 46期号:8页码:683-685 |
| 关键词 | EGF receptor tyrosine kinase nonradioisotopic GST in vitro |
| 通讯作者 | Lin, QS (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | A non-radioisotopic method was developed for the assay of epidermal growth factor receptor (EGFR), A peptide with twenty amino acid residues around Tyr 1173, the major phosphorylation site of EGFR, was cloned as a GST fusion protein and used as substrate. Anti-phosphotyrosine monoclonal antibody PY99 was used for the determination of the extent of phosphorylation. Both the specificity and the sensitivity were substantially higher than that of the existing method. K-m value of the fusion protein is much lower (10 mu mol/L) than that of the synthetic peptide (110 mu mol/L). The method can be applied to the measurement of the tyrosine kinase activity of c-erb B2 (Neu/HER2). |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | EPIDERMAL GROWTH-FACTOR ; POINT MUTATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000168737900019 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2700]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ge, GX,Wu, J,Lin, QS. Non-radioisotopic method for the in vitro measurement of EGF receptor tyrosine kinase[J]. CHINESE SCIENCE BULLETIN,2001,46(8):683-685. |
| APA | Ge, GX,Wu, J,&Lin, QS.(2001).Non-radioisotopic method for the in vitro measurement of EGF receptor tyrosine kinase.CHINESE SCIENCE BULLETIN,46(8),683-685. |
| MLA | Ge, GX,et al."Non-radioisotopic method for the in vitro measurement of EGF receptor tyrosine kinase".CHINESE SCIENCE BULLETIN 46.8(2001):683-685. |
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