Propranolol increases phosphatidic acid level via activation of phospholipase D
文献类型：期刊论文
| 作者 | Chai, MQ; Chen, JS; Zhao, S; Song, JG; Chai, MQ; Chen, JS; Zhao, S; Song, JG |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 22期号:9页码:777-784 |
| 关键词 | phospholipase D phosphatidic acids protein kinase C propranolol staurosporine A-549 cells immunoblotting |
| 通讯作者 | Song, JG (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | AIM: To investigate the propranolol-induced phospholipase D (PLD) activity, its contribution to the increase in the level of phosphatidic acid, and the role of protein kinase C (PKC) in this event. METHODS: A combination of [H-3]-myristate labeling, transphosphatidylation reaction, lipid extraction, and d-iin layer chromatography was used to measure the PLD activity. PKC inhibitors and prolonged phorbol-12-myristate-13-acetate (PMA) treatment were used to study the involvement of PKC in propranolol-induced PLD activation. Immunoblotting was used to detect the intracellular levels of PKC. RESULTS: Treatment of A-549 cells with propranolol in the presence of butanol, resulted in the rapid activation of PLD. Propranolol induced the formation of phosphatidylbutanol (PBut), a unique product of PLD, at the expense of phosphatidic acid (PA) formation. Pretreatment of cells with PKC inhibitors Ro-31-8220, staurosporine, and rottlerin increased the propranolol-induced PLD. Down-regulation of PKC by prolonged treatment of cells with PMA also potentiated the propranolol-induced PLD activity. CONCLUSION: Propranolol induces rapid activation of PLD activity, which results in the increase in intracellular level of PA. The data also indicate that propranolol-induced PLD activity could be negatively regulated by PKC. |
| 学科主题 | Chemistry ; Pharmacology & Pharmacy |
| 类目[WOS] | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 关键词[WOS] | PROTEIN-KINASE-C ; EPIDERMAL GROWTH-FACTOR ; ENDOTHELIAL-CELLS ; PLASMA-MEMBRANE ; PHORBOL ESTERS ; DIACYLGLYCEROL ; PHOSPHOHYDROLASE ; OVEREXPRESSION ; STIMULATION ; INVOLVEMENT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000171146200002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2701]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chai, MQ,Chen, JS,Zhao, S,et al. Propranolol increases phosphatidic acid level via activation of phospholipase D[J]. ACTA PHARMACOLOGICA SINICA,2001,22(9):777-784. |
| APA | Chai, MQ.,Chen, JS.,Zhao, S.,Song, JG.,Chai, MQ.,...&Song, JG.(2001).Propranolol increases phosphatidic acid level via activation of phospholipase D.ACTA PHARMACOLOGICA SINICA,22(9),777-784. |
| MLA | Chai, MQ,et al."Propranolol increases phosphatidic acid level via activation of phospholipase D".ACTA PHARMACOLOGICA SINICA 22.9(2001):777-784. |
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