Anti-human hepatocellular carcinoma effects of tumor necrosis factor-related apoptosis-inducing ligand in vivo and in vitro
文献类型：期刊论文
| 作者 | Guo, BC; Xu, YH |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 22期号:9页码:831-836 |
| 关键词 | apoptosis bcl-2 genes tumor necrosis factor membrane glycoproteins hepatocellular carcinoma molecular cloning reverse transcriptase polymerase chain reaction |
| 通讯作者 | Xu, YH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | AIM: To investigate the effect of over-expression of Bcl-2 protein on Trail protein-induced apoptosis in human hepatoma cells, and the cytotoxicity of Trail protein on human hepatoma cells in vitro and in vivo. METHODS: The Trail gene was cloned and expressed in E coli. The cytotoxicity of the recombinant Trail protein was assayed on human hepatoma cells in vitro and in vivo. The cell viability was assessed by trypan blue exclusion. The stable human hepatoma cells clone in which Bcl-2 protein over-expressed was established by transfecting eukaryotic expression plasmid pcDNA3-Bcl-2 into BEL-74-04 human hepatoma cells, and was selected with G418 400 mg (.) L-1. RESULTS: The recombinant Trail protein actively killed human hepatoma cells tested in this study such as BEL-7404, BEL-7402, and SMMC-7721. Over-expression of Bcl-2 protein could inhibit apoptosis induced by Trail in BEL-7404 human hepatoma cells in vitro. It was obvious that the purified recombinant Trail protein could inhibit tumor formation of BEL-7404. human hepatoma cells in nude mice. CONCLUSION: The recombinant Trail protein could kill human hepatoma cells in vitro and in vivo. Overexpression of Bcl-2 protein could inhibit Trail-induced apoptosis in BEL-7404. human hepatoma cells. The results suggested that Trail might be a potential agent for the liver cancer therapy. |
| 学科主题 | Chemistry; Pharmacology & Pharmacy |
| 类目[WOS] | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 关键词[WOS] | TRAIL-INDUCED APOPTOSIS ; DEATH DOMAIN ; RECEPTOR SUPERFAMILY ; IN-VIVO ; FAMILY ; MEMBER ; CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000171146200012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2702]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Guo, BC,Xu, YH. Anti-human hepatocellular carcinoma effects of tumor necrosis factor-related apoptosis-inducing ligand in vivo and in vitro[J]. ACTA PHARMACOLOGICA SINICA,2001,22(9):831-836. |
| APA | Guo, BC,&Xu, YH.(2001).Anti-human hepatocellular carcinoma effects of tumor necrosis factor-related apoptosis-inducing ligand in vivo and in vitro.ACTA PHARMACOLOGICA SINICA,22(9),831-836. |
| MLA | Guo, BC,et al."Anti-human hepatocellular carcinoma effects of tumor necrosis factor-related apoptosis-inducing ligand in vivo and in vitro".ACTA PHARMACOLOGICA SINICA 22.9(2001):831-836. |
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