Structural transformation of the peptide fragments from the reactive center loops of serpins: Circular dichroic studies
文献类型：期刊论文
| 作者 | Hu, HY; Chen, YN; Xu, SQ; Cu, GJ |
| 刊名 | CHINESE JOURNAL OF CHEMISTRY
 |
| 出版日期 | 2001 |
| 卷号 | 19期号:10页码:954-959 |
| 关键词 | serpin reactive center circular dichroism structural transformation |
| 通讯作者 | Hu, HY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | Two peptides, derived from the reactive center of ovalbumin.(OVARC) and plasminogen activator inhibitor-1 (PAIRC) respectively were chemically synthesized and investigated by circular dichroic spectroscopy. The secondary structural transformation in solution and in solid state was studied. OVARC shows a nascent helical structure in aqueous solution, and its helical content increases under acidic conditions. There is no obvious structural conversion from solution to solid state. PAIRC, however, undergoes a structural transformation from random coil in aqueous solution to a typical beta -sheet structure in the solid state. Hexafluoroispropanol (HFIP) prompts helical structures of the two peptides in solution, but It seems to trigger the structural formation of beta -sheets in solid state. The novel structural transformation from random coil or nascent helical structure in aqueous solution to the alpha -helix in IMP and to the beta -sheet structure in solid state may reflect the conformational polymorphism of the serpin reactive centers and is implicated in the structural features of the amyloid aggregates. |
| 学科主题 | Chemistry |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 关键词[WOS] | CRYSTAL-STRUCTURE ; OVALBUMIN ; PROTEIN ; DISEASE ; CONFORMATION ; TCS-182-200 ; MODEL ; HEAT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000171955100007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2704]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, HY,Chen, YN,Xu, SQ,et al. Structural transformation of the peptide fragments from the reactive center loops of serpins: Circular dichroic studies[J]. CHINESE JOURNAL OF CHEMISTRY,2001,19(10):954-959. |
| APA | Hu, HY,Chen, YN,Xu, SQ,&Cu, GJ.(2001).Structural transformation of the peptide fragments from the reactive center loops of serpins: Circular dichroic studies.CHINESE JOURNAL OF CHEMISTRY,19(10),954-959. |
| MLA | Hu, HY,et al."Structural transformation of the peptide fragments from the reactive center loops of serpins: Circular dichroic studies".CHINESE JOURNAL OF CHEMISTRY 19.10(2001):954-959. |
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