Intracellular inhibition of the replication of hepatitis B virus by hammerhead ribozymes
文献类型：期刊论文
| 作者 | Feng, Y; Kong, YY; Wang, Y; Qi, GR |
| 刊名 | JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
 |
| 出版日期 | 2001 |
| 卷号 | 16期号:10页码:1125-1130 |
| 关键词 | antiviral core protein DNA replication hammerhead ribozyme hepatitis B virus |
| 通讯作者 | Qi, GR (reprint author), Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China., |
| 英文摘要 | Background: Chronic hepatitis B virus (HBV) infection is frequently associated with cirrhosis and hepatocellular carcinoma, and so has become a major worldwide health problem. Hammerhead ribozymes have recently gained some attention as potential tools to inhibit viral infection, for which there are no general effective therapies available. Methods: A hammerhead ribozyme, RzC, was designed to target the sequence encoding the tail region of the HBV core protein. The activities of the ribozyme were analyzed in vitro and in human hepatoma (HepG2) cells. Results: In vitro, RzC cleaves HBV-RNA at its target site up to 30%, while the disabled ribozyme, dRzC, which has a one-base mutation in the catalytic site, did not cleave the target RNA at all. When the ribozymes were cotransfected into HepG2 cells with the HBV genome-containing plasmid, p3.6II, the inhibition of HBV replication by RzC was greater than that by dRzC, indicating that the active catalytic domain of the hammerhead ribozyme could increase the extent of antisense-mediated inhibition. In addition, there was a gradient of effectiveness in which the greater the amount of released ribozyme, the greater the reduction in HBV progeny DNA. Conclusions: These results suggest the possibility of hammerhead ribozyme-mediated gene therapy for the treatment of HBV infections. (C) 2001 Blackwell Science Asia Pty Ltd. |
| 学科主题 | Gastroenterology & Hepatology |
| 类目[WOS] | Gastroenterology & Hepatology |
| 关键词[WOS] | CORE PROTEIN ; CLEAVAGE ; PHOSPHORYLATION ; SIGNAL ; CELLS ; RNA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000179450900009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2706]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Feng, Y,Kong, YY,Wang, Y,et al. Intracellular inhibition of the replication of hepatitis B virus by hammerhead ribozymes[J]. JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY,2001,16(10):1125-1130. |
| APA | Feng, Y,Kong, YY,Wang, Y,&Qi, GR.(2001).Intracellular inhibition of the replication of hepatitis B virus by hammerhead ribozymes.JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY,16(10),1125-1130. |
| MLA | Feng, Y,et al."Intracellular inhibition of the replication of hepatitis B virus by hammerhead ribozymes".JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY 16.10(2001):1125-1130. |
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