Proteome alterations in human hepatoma cells transfected with antisense epidermal growth factor receptor sequence
文献类型：期刊论文
| 作者 | Yu, LR; Shao, XX; Jiang, WL; Xu, D; Chang, YC; Xu, YH; Xia, QC |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2001 |
| 卷号 | 22期号:14页码:3001-3008 |
| 关键词 | proteome two-dimensional gel electrophoresis mass spectrometry human hepatoma cells epidermal growth factor receptor |
| 通讯作者 | Xia, QC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | The epidermal growth factor (EGF) is a member of the growth factor superfamily that can stimulate the proliferation of many types of cells. Overexpression of EGF receptor (EGFR) was observed in many types of cancer cells. Anti-EGFR antibodies or antisense nucleic acid sequences of EGFR can suppress the growth of hepatoma cells. In order to further investigate the proteome alterations associated with malignant growth of the human hepatoma cells and the influence of EGFR signal pathway on the cellular proteome, we have comparatively analyzed the proteomes of human hepatoma cells transfected with antisense EGFR sequence (cell strain JX-1) and its control cells (cell strain JX-0) by two-dimensional (2-D) gel electrophoresis and mass spectrometry. Image analysis of silver-stained 2-D gels revealed that 40 protein spots showed significant expression changes in JX-1 cells compared to JX-0 cells. Three of them, including the tumor suppressor protein maspin, changed with tendency to the normal levels. Two protein spots were identified as HSP27 in the same gel, and one of them had a,reduced level in JX-1 cells. The apparent alterations of HSP27 in expression level might be the results from their differential chemical modifications, suggesting the eff ect of dynamic post-translational modifications of proteins on the growth of hepatoma cells. Other proteins such as glutathione peroxidase (GPX-1) and 14-3-3-sigma also exhibited altered expression in JX-1 cells, and their functional implications are discussed. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 关键词[WOS] | 2-DIMENSIONAL GEL-ELECTROPHORESIS ; HUMAN HEPATOCELLULAR-CARCINOMA ; IMMOBILIZED PH GRADIENTS ; MASS-SPECTROMETRY ; GLUTATHIONE-PEROXIDASE ; GENE-EXPRESSION ; CANCER-CELLS ; EGF-RECEPTOR ; PROTEINS ; MASPIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000170920900019 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2708]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yu, LR,Shao, XX,Jiang, WL,et al. Proteome alterations in human hepatoma cells transfected with antisense epidermal growth factor receptor sequence[J]. ELECTROPHORESIS,2001,22(14):3001-3008. |
| APA | Yu, LR.,Shao, XX.,Jiang, WL.,Xu, D.,Chang, YC.,...&Xia, QC.(2001).Proteome alterations in human hepatoma cells transfected with antisense epidermal growth factor receptor sequence.ELECTROPHORESIS,22(14),3001-3008. |
| MLA | Yu, LR,et al."Proteome alterations in human hepatoma cells transfected with antisense epidermal growth factor receptor sequence".ELECTROPHORESIS 22.14(2001):3001-3008. |
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