A novel mechanism of dopamine neurotoxicity involving the peripheral extracellular and the plasma membrane dopamine transporter
文献类型：期刊论文
| 作者 | Liu, Z; Zhang, J; Fei, J; Guo, LH |
| 刊名 | NEUROREPORT
 |
| 出版日期 | 2001 |
| 卷号 | 12期号:15页码:3293-3297 |
| 关键词 | apoptosis cell death CHO cells dopamine dopamine transporter forskolin SK-N-SH cells |
| 通讯作者 | Guo, LH (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China., |
| 英文摘要 | Chinese hamster ovary cells stably expressing a rat dopamine transporter (designated D8 cells) and neuroblastoma SK-N-SH cells were used as two model systems to study dopamine neurotoxicity. Within 24 h, 1-10 mM dopamine induced D8 cells into apoptosis while 20-200 muM dopamine induced SK-N-SH cells into cell death. The viability of both cell types decreased in a dose-dependent manner. However, the dopamine uptake activity of D8 cells at 10 mM was not significantly higher than the uptake at 100 muM, suggesting that it was not the high concentration of intracellular dopamine that induced D8 cells into apoptosis, but rather dopamine found in the extracellular space. Furthermore, cocaine, an inhibitor of dopamine uptake, could not block cell death induced by dopamine. Forskolin, an agonist of protein kinase A (PKA), stimulated dopamine uptake in D8 cells and blocked apoptosis induced by the drug. These results suggest that the dopamine transporter mediates a dopamine-dependant apoptotic signal transduction pathway that is independent of dopamine uptake into the cell. NeuroReport 12:3293-3297 (C) 2001 Lippincott Williams & Wilkins. |
| 学科主题 | Neurosciences & Neurology |
| 类目[WOS] | Neurosciences |
| 关键词[WOS] | CENTRAL-NERVOUS-SYSTEM ; HUMAN NEURONAL CELLS ; PARKINSONS-DISEASE ; INDUCED APOPTOSIS ; DEATH ; GLUTATHIONE ; TOXICITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000171902600025 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2709]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Z,Zhang, J,Fei, J,et al. A novel mechanism of dopamine neurotoxicity involving the peripheral extracellular and the plasma membrane dopamine transporter[J]. NEUROREPORT,2001,12(15):3293-3297. |
| APA | Liu, Z,Zhang, J,Fei, J,&Guo, LH.(2001).A novel mechanism of dopamine neurotoxicity involving the peripheral extracellular and the plasma membrane dopamine transporter.NEUROREPORT,12(15),3293-3297. |
| MLA | Liu, Z,et al."A novel mechanism of dopamine neurotoxicity involving the peripheral extracellular and the plasma membrane dopamine transporter".NEUROREPORT 12.15(2001):3293-3297. |
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