Site-specific uv crosslinking of minihelix DNA and TrpRS from Bacillus subtilis
文献类型：期刊论文
| 作者 | Xu, F; Jiang, G; Zhang, Y; Zhu, LF; Jin, YX; Wang, DB |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2001 |
| 卷号 | 46期号:16页码:1380-1383 |
| 关键词 | uv crosslinking minihelix DNA TrpRS discriminator |
| 通讯作者 | Jin, YX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | In order to investigate the recognition mechanism and the relationship between structure and function of minihelix DNA with Tryptophanyl-tRNA Synthetase (TrpRS), TrpRS from Bacillus Subtilis was purified. Four minihelix DNAs were chemically synthesized and the photoreactive reagent s(4)T was incorporated into three of them at the positions of G73, T72 and T55 corresponding to tRNA(Trp). The apparatus for uv crosslinking was devised and the parameters for uv crosslinking were optimized. The results indicated that the G73 and T72 base of minihelix DNA interacted with TrpRS directly. The uv crosslinking reaction was improved by the dose of uv irradiation and the concentration of both TrpRS and minihelix DNA. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | TRANSFER RNA-SYNTHETASE ; RECOGNITION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000171106700013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2711]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xu, F,Jiang, G,Zhang, Y,et al. Site-specific uv crosslinking of minihelix DNA and TrpRS from Bacillus subtilis[J]. CHINESE SCIENCE BULLETIN,2001,46(16):1380-1383. |
| APA | Xu, F,Jiang, G,Zhang, Y,Zhu, LF,Jin, YX,&Wang, DB.(2001).Site-specific uv crosslinking of minihelix DNA and TrpRS from Bacillus subtilis.CHINESE SCIENCE BULLETIN,46(16),1380-1383. |
| MLA | Xu, F,et al."Site-specific uv crosslinking of minihelix DNA and TrpRS from Bacillus subtilis".CHINESE SCIENCE BULLETIN 46.16(2001):1380-1383. |
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