中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Eukaryotic elongation factor 2 can bind to the synthetic oligoribonucleotide that mimics sarcin/ricin domain of rat 28S ribosomal RNA

文献类型:期刊论文

作者Tang, S; He, WJ; Xu, H; Liu, WY; Ruan, KC
刊名MOLECULAR AND CELLULAR BIOCHEMISTRY
出版日期2001
卷号223期号:1页码:117-121
关键词ADP-ribosylation eukaryotic elongation factor 2 (eEF2) ribosomal RNA sarcin/ricin domain
通讯作者Liu, WY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to P site by binding to the ribosome. In this work, the complex formation of rat liver eEF2 with a synthetic oligoribonucleotide (SRD RNA) that mimics sarcin/ricin domain of rat 28S ribosomal RNA is invested in vitro. Purified eEF2 can specifically bind SRD RNA to form a stable complex. tRNA competes with SRD RNA in binding to eEF2 in a less extent. Pretreatment of eEF2 with GDP or ADP-ribosylation of eEF2 by diphtheria toxin can obviously reduce the ability of eEF2 to form the complex with the synthetic oligoribonucleotide. These results indicate that eEF2 is likely to bind directly to the sarcin/ricin domain of 28S ribosomal RNA in the process of protein synthesis.
学科主题Cell Biology
类目[WOS]Cell Biology
关键词[WOS]FACTOR-II ; ADP-RIBOSYLATION ; DIPHTHERIA-TOXIN ; PROTEIN ; TRANSLOCATION ; MECHANISM
收录类别SCI
语种英语
WOS记录号WOS:000170948100014
版本出版稿
源URL[http://202.127.25.143/handle/331003/2723]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714
Tang, S,He, WJ,Xu, H,et al. Eukaryotic elongation factor 2 can bind to the synthetic oligoribonucleotide that mimics sarcin/ricin domain of rat 28S ribosomal RNA[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2001,223(1):117-121.
APA Tang, S,He, WJ,Xu, H,Liu, WY,&Ruan, KC.(2001).Eukaryotic elongation factor 2 can bind to the synthetic oligoribonucleotide that mimics sarcin/ricin domain of rat 28S ribosomal RNA.MOLECULAR AND CELLULAR BIOCHEMISTRY,223(1),117-121.
MLA Tang, S,et al."Eukaryotic elongation factor 2 can bind to the synthetic oligoribonucleotide that mimics sarcin/ricin domain of rat 28S ribosomal RNA".MOLECULAR AND CELLULAR BIOCHEMISTRY 223.1(2001):117-121.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

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