Eukaryotic elongation factor 2 can bind to the synthetic oligoribonucleotide that mimics sarcin/ricin domain of rat 28S ribosomal RNA
文献类型：期刊论文
| 作者 | Tang, S; He, WJ; Xu, H; Liu, WY; Ruan, KC |
| 刊名 | MOLECULAR AND CELLULAR BIOCHEMISTRY
 |
| 出版日期 | 2001 |
| 卷号 | 223期号:1页码:117-121 |
| 关键词 | ADP-ribosylation eukaryotic elongation factor 2 (eEF2) ribosomal RNA sarcin/ricin domain |
| 通讯作者 | Liu, WY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to P site by binding to the ribosome. In this work, the complex formation of rat liver eEF2 with a synthetic oligoribonucleotide (SRD RNA) that mimics sarcin/ricin domain of rat 28S ribosomal RNA is invested in vitro. Purified eEF2 can specifically bind SRD RNA to form a stable complex. tRNA competes with SRD RNA in binding to eEF2 in a less extent. Pretreatment of eEF2 with GDP or ADP-ribosylation of eEF2 by diphtheria toxin can obviously reduce the ability of eEF2 to form the complex with the synthetic oligoribonucleotide. These results indicate that eEF2 is likely to bind directly to the sarcin/ricin domain of 28S ribosomal RNA in the process of protein synthesis. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | FACTOR-II ; ADP-RIBOSYLATION ; DIPHTHERIA-TOXIN ; PROTEIN ; TRANSLOCATION ; MECHANISM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000170948100014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2723]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Tang, S,He, WJ,Xu, H,et al. Eukaryotic elongation factor 2 can bind to the synthetic oligoribonucleotide that mimics sarcin/ricin domain of rat 28S ribosomal RNA[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2001,223(1):117-121. |
| APA | Tang, S,He, WJ,Xu, H,Liu, WY,&Ruan, KC.(2001).Eukaryotic elongation factor 2 can bind to the synthetic oligoribonucleotide that mimics sarcin/ricin domain of rat 28S ribosomal RNA.MOLECULAR AND CELLULAR BIOCHEMISTRY,223(1),117-121. |
| MLA | Tang, S,et al."Eukaryotic elongation factor 2 can bind to the synthetic oligoribonucleotide that mimics sarcin/ricin domain of rat 28S ribosomal RNA".MOLECULAR AND CELLULAR BIOCHEMISTRY 223.1(2001):117-121. |
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