Identification and characterization of a novel human cDNA encoding a 21 kDa pRb-associated protein
文献类型：期刊论文
| 作者 | Wen, H; Ao, SZ |
| 刊名 | GENE
 |
| 出版日期 | 2001 |
| 卷号 | 263期号:1页码:85-92 |
| 关键词 | yeast two-hybrid system RBP21 pRb-associated protein in vitro binding in vivo binding RH mapping subcellular localization |
| 通讯作者 | Ao, SZ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | The retinoblastoma protein (pRb) functions as a critical master regulator in cell cycle regulation, which is an important cell-regulatory process, through its interaction with various cellular proteins. Using the C-terminus of human pRb and the yeast two-hybrid system, a novel protein named RBP21 that contains 187 amino acid residues with a calculated molecular weight of 21 kDa was identified as a pRb-binding protein. Sequence analysis indicates that RBP21 shares homology with other retinoblastoma-binding proteins in the pRb-binding motif LxCxE at the C-terminal region. In vitro specific interaction between pRb and RBP21 was confirmed using in vitro translation products. When overexpressed in COS-7 cells, RBP21 could co-immunoprecipitate with pRb. This interaction requires the LxCxE motif of RBP21 and the entire pocket region of pRb. Each point mutation of the conserved amino acid residues in pRb-binding motif of RBP21 abolished its specific interaction with pRb. RH mapping result showed that this novel gene was mapped to chromosome region 15q21.1-21.3. Northern blot analysis suggested that RBP21 was widely expressed in various human tissues and cancer cell lines. When expressed in HeLa cells as a green fluorescent protein fusion, RBP21 was distributed throughout the cell. (C) 2001 Elsevier Science B.V. All rights reserved. |
| 学科主题 | Genetics & Heredity |
| 类目[WOS] | Genetics & Heredity |
| 关键词[WOS] | GREEN FLUORESCENT PROTEIN ; RETINOBLASTOMA PROTEIN ; CELL-CYCLE ; GENE-PRODUCT ; DIFFERENTIATION ; TRANSCRIPTION ; FAMILY ; DNA ; EXPRESSION ; REPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000167352400009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2726]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wen, H,Ao, SZ. Identification and characterization of a novel human cDNA encoding a 21 kDa pRb-associated protein[J]. GENE,2001,263(1):85-92. |
| APA | Wen, H,&Ao, SZ.(2001).Identification and characterization of a novel human cDNA encoding a 21 kDa pRb-associated protein.GENE,263(1),85-92. |
| MLA | Wen, H,et al."Identification and characterization of a novel human cDNA encoding a 21 kDa pRb-associated protein".GENE 263.1(2001):85-92. |
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