Exploration of RNA 3 ' terminal locations in rat ribosome
文献类型：期刊论文
| 作者 | Gao, X; Ruan, KC; Liu, WY |
| 刊名 | MOLECULAR AND CELLULAR BIOCHEMISTRY
 |
| 出版日期 | 2001 |
| 卷号 | 225期号:1页码:161-165 |
| 关键词 | 3 ' end of rRNA fluorescence-labeling rat ribosome |
| 通讯作者 | Ruan, KC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | The locations of the 3' ends of RNAs in rat ribosome were studied by a fluorescence-labeling method combined with high hydrostatic pressure and agarose electrophoresis. Under physiological conditions, only the 3' ends of 28 S and 5.8 S RNA in 80 S ribosome could be labeled with a high sensitive fluorescent probe - fluorescein 5-thiosemicarbazide (FTSC), indicating that the 3' termini of 28 S and 5.8 S RNA were located on or near the surface of 80 S ribosome. The 3' terminus of 5 S RNA could be attacked by FTSC only in the case of the dissociation of the 80 S ribosome into two subunits induced by high salt concentration (1 M KCl) or at high hydrostatic pressure, showing that the 3' end of 5 S RNA was located on the interface of two subunits. However, no fluorescence-labeled 18 S RNA could be detected under all the conditions studied, suggesting that the 3' end of 18 S RNA was either located deeply inside ribosome or on the surface but protected by proteins. It was interesting to note that modifications of the 3' ends of ribosomal RNAs including oxidation with NaIO4, reduction with KBH4 and labeling with fluorescent probe did not destroy the translation activity of ribosome, indicating that the 3' ends of RNAs were not involved in the translation activity of ribosome. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | POLYACRYLAMIDE-GEL ; SUBUNIT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000171402500019 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2727]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Gao, X,Ruan, KC,Liu, WY. Exploration of RNA 3 ' terminal locations in rat ribosome[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2001,225(1):161-165. |
| APA | Gao, X,Ruan, KC,&Liu, WY.(2001).Exploration of RNA 3 ' terminal locations in rat ribosome.MOLECULAR AND CELLULAR BIOCHEMISTRY,225(1),161-165. |
| MLA | Gao, X,et al."Exploration of RNA 3 ' terminal locations in rat ribosome".MOLECULAR AND CELLULAR BIOCHEMISTRY 225.1(2001):161-165. |
入库方式： OAI收割
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