Antisense of ATP synthase subunit e inhibits the growth of human hepatocellular carcinoma cells
文献类型：期刊论文
| 作者 | Ying, H; Yu, YL; Xu, YH |
| 刊名 | ONCOLOGY RESEARCH
 |
| 出版日期 | 2001 |
| 卷号 | 12期号:1页码:485-490 |
| 关键词 | differential display hepatocellular carcinoma ATP synthase subunit e growth inhibition mitogen-activated protein kinase |
| 通讯作者 | Xu, YH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Mol & Cellular Oncol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Differentially expressed genes between normal and hepatocellular carcinoma tissues were investigated using differential display. We identified a cDNA fragment that was overexpressed in cancer tissue. Homology analysis showed that the sequence was identical to human ATP synthase subunit e (hAS-e). Moreover, Northern blot analysis demonstrated that hAS-e was overexpressed in 10 of 11 (91%) specimens of hepatocellular carcinoma compared with corresponding normal tissues. We introduced antisense hAS-e into a human hepatocellular carcinoma BEL-7404 cell and found that downregulation of the hAS-e led to cell growth inhibition. It was also found that the antisense transfection could decrease the serum-stimulated activation of mitogen-activated protein kinase (MAP kinase). Together, the results suggest that antisense of hAS-e can inhibit cell proliferation through the MAP kinase pathway. Our data indicate that hAS-e may become a new target in gene therapy. |
| 学科主题 | Oncology |
| 类目[WOS] | Oncology |
| 关键词[WOS] | BOVINE HEART-MITOCHONDRIA ; AMINO-ACID-SEQUENCE ; ASCITES TUMOR-CELLS ; CONSUMING PROCESSES ; CANCER ; PURIFICATION ; ACCUMULATION ; TOXICITY ; CYCLE ; DYE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000174533800006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2730]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ying, H,Yu, YL,Xu, YH. Antisense of ATP synthase subunit e inhibits the growth of human hepatocellular carcinoma cells[J]. ONCOLOGY RESEARCH,2001,12(1):485-490. |
| APA | Ying, H,Yu, YL,&Xu, YH.(2001).Antisense of ATP synthase subunit e inhibits the growth of human hepatocellular carcinoma cells.ONCOLOGY RESEARCH,12(1),485-490. |
| MLA | Ying, H,et al."Antisense of ATP synthase subunit e inhibits the growth of human hepatocellular carcinoma cells".ONCOLOGY RESEARCH 12.1(2001):485-490. |
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