Expression, purification, immunological characterization and application of Escherichia coli-derived hepatitis C virus E2 proteins
文献类型：期刊论文
| 作者 | Liu, J; Zhu, LX; Zhang, XX; Lu, M; Kong, YY; Wang, Y; Li, GD |
| 刊名 | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
 |
| 出版日期 | 2001 |
| 卷号 | 34期号:1页码:109-119 |
| 关键词 | hexahistidine-tagged protein humoral immune response patients' sera vaccine |
| 通讯作者 | Wang, Y (reprint author), Chinese Acad Sci, Shanghai Inst Life Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | The envelope glycoprotein E2 of hepatitis C virus (HCV) has been shown to bind human target cells. Anti-E2 antibodies have been associated with both recovery from natural infection in humans and protection from challenge with homologous HCV in chimpanzees. Therefore E2 has become a major target for the development of anti-HCV vaccines. Two E2 fragments [amino acids (aa) 450-565 and aa 385-565] derived from a subtype 1b HCV genome were expressed as N-terminally hexahistidine-tagged proteins in Escherichia coli and purified to over 85% purity. Both proteins were specifically recognized by homologous hepatitis-C-patient's serum on Western blotting, suggesting that these E. coli-derived E2 proteins displayed E2-specific antigenicity. E2-116 (aa 450-565) elicited strong antibody responses in BALB/c mice and rabbits. Rabbit antiserum raised against renatured E2-116 (RE2-116R) was able to recognize subtype lb and [a E2 glycoproteins expressed in mammalian cells on Western blotting. E2-181 (aa 385-56S) reacted with 40% of anti-HCV+ patients' sera in ELISA. RE2-116R and E2-181 were successfully used in preliminary modified vaccinia virus Ankara- and DNA-based E2 vaccine studies for detecting antigen expression in vitro and assessing induced humoral immune responses in mice. The E2 proteins and rabbit antiserum reported here could find wider applications in the development of effective diagnostic, prophylactic and therapeutic measures against HCV. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | ANTI-HCV ; GLYCOPROTEIN ; ANTIBODIES ; CELLS ; INFECTION ; ENVELOPE ; BINDING ; IDENTIFICATION ; PRODUCTS ; GENOME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000171916700007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2731]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, J,Zhu, LX,Zhang, XX,et al. Expression, purification, immunological characterization and application of Escherichia coli-derived hepatitis C virus E2 proteins[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2001,34(1):109-119. |
| APA | Liu, J.,Zhu, LX.,Zhang, XX.,Lu, M.,Kong, YY.,...&Li, GD.(2001).Expression, purification, immunological characterization and application of Escherichia coli-derived hepatitis C virus E2 proteins.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,34(1),109-119. |
| MLA | Liu, J,et al."Expression, purification, immunological characterization and application of Escherichia coli-derived hepatitis C virus E2 proteins".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 34.1(2001):109-119. |
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