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Three-hybrid strategy reveals a peptide segment that specifically binds to the 3 '-untranslated region of NF-IL6 mRNA
文献类型：期刊论文
| 作者 | He, YZ; Liu, DG |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2000 |
| 卷号 | 276期号:1页码:101-106 |
| 关键词 | NF-IL6 3 ' UTR RNA-binding protein yeast three-hybrid system |
| 通讯作者 | Liu, DG (reprint author), Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | The 3'UTR of eukaryotic mRNA is an important regulation region, on which many trans factors act. In recent years, a series of 3'UTRs were shown, to have tumor suppressor function, including the 3'UTR of the human nuclear factor for interleukin-6 (NF-IL6 3'UTR). To understand molecular basis for this function, we have tried to isolate genes encoding protein factors acting on the RNA of NF-IL6 3'UTR. Here we show that, by using a yeast three-hybrid system, a cDNA fragment was successfully isolated. This cDNA was allowed to express in E. coli, and its expression product, a polypeptide of ca. 70 amino acids long, was shown to specifically bind to the NF-IL6 3'UTR RNA. A search in GenBank did not reveal homologous sequences. Therefore, this cDNA fragment may be a part of the gene of a novel NF-IL6 3'UTR specific binding protein. (C) 2000 Academic Press. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | MESSENGER-RNA ; PROTEIN ; ELEGANS ; CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000089388100019 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2737]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | He, YZ,Liu, DG. Three-hybrid strategy reveals a peptide segment that specifically binds to the 3 '-untranslated region of NF-IL6 mRNA[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2000,276(1):101-106. |
| APA | He, YZ,&Liu, DG.(2000).Three-hybrid strategy reveals a peptide segment that specifically binds to the 3 '-untranslated region of NF-IL6 mRNA.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,276(1),101-106. |
| MLA | He, YZ,et al."Three-hybrid strategy reveals a peptide segment that specifically binds to the 3 '-untranslated region of NF-IL6 mRNA".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 276.1(2000):101-106. |
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