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Induction of Ref-1 ensures AP-1 activation in intracellular oxidative environment of IL-2-stimulated BA/F3 beta cells
文献类型：期刊论文
| 作者 | Yan, MD; Xu, WJ; Lu, LR; Sun, LY; Liu, XY; Zheng, ZC |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2000 |
| 卷号 | 278期号:2页码:462-469 |
| 关键词 | interleukin-2 (IL-2) redox factor-1 (Ref-1) activator protein-1 (AP-1) intracellular environment redox regulation |
| 通讯作者 | Zheng, ZC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Our previous study of interleukin-2 (IL-2) signaling found that redox factor-1 (Ref-l) mRNA was upregulated by IL-2. in this study,we further studied the function of Ref-l in the potential redox regulation of IL-2 signaling in BA/F3 beta cells. Western blot analysis confirmed that IL-2 stimulation increases Ref-l protein. Flow cytometric assay by using 2',7'-dichlorofluorescin diacetate indicated that IL-2 stimulation results in an oxidative shift of intracellular environment. However, IL-2-induced activator protein-1 (AP-1) is oxidation-sensitive. Gel shift assays of nuclear extracts immunodepleted of Ref-l protein demonstrated that IL-2-induced AP-1 DNA binding is dependent on the presence of Ref-l. This was further confirmed by the restoration of AP-1 DNA binding upon the re-addition of immunoprecipitated Ref-l. Additionally, reporter gene assays showed that AP-1 transcriptional activity was enhanced by the overexpression of Ref-l and attenuated by the introduction of antisense Ref-l. These results suggest that the induction of Ref-l ensures AP-1 activation in the intracellular oxidative environment of IL-2-stimulated BA/F3 beta cells, (C) 2000 Academic Press. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | DNA-BINDING ACTIVITY ; REDOX REGULATION ; FREE-RADICALS ; NITRIC-OXIDE ; REPAIR ENZYME ; JUN ; PROTEIN ; IDENTIFICATION ; INTERLEUKIN-2 ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000165610700031 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2740]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yan, MD,Xu, WJ,Lu, LR,et al. Induction of Ref-1 ensures AP-1 activation in intracellular oxidative environment of IL-2-stimulated BA/F3 beta cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2000,278(2):462-469. |
| APA | Yan, MD,Xu, WJ,Lu, LR,Sun, LY,Liu, XY,&Zheng, ZC.(2000).Induction of Ref-1 ensures AP-1 activation in intracellular oxidative environment of IL-2-stimulated BA/F3 beta cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,278(2),462-469. |
| MLA | Yan, MD,et al."Induction of Ref-1 ensures AP-1 activation in intracellular oxidative environment of IL-2-stimulated BA/F3 beta cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 278.2(2000):462-469. |
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