Promoting effect of IFN-gamma on the expression of LPS-induced IL-12 p40 and p35 mRNA in murine suppressor macrophages
文献类型：期刊论文
| 作者 | Ql, J; Zhang, JS; Feng, WG; Luo, XD; Li, CL; Zhang, ZL |
| 刊名 | SCIENCE IN CHINA SERIES C-LIFE SCIENCES
 |
| 出版日期 | 2000 |
| 卷号 | 43期号:6页码:578-588 |
| 关键词 | IL-12 p40/p35 LPS suppressor macrophage IFN-gamma NF-kappa B |
| 通讯作者 | Zhang, ZL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | We detected the expression of IL-12 p40/p35 mRNA by semi-quantitative RT-PCR and silver staining, and studied the molecular interaction between the IL-12 expression and the NF-kappaB activation induced by LPS and IFN-gamma /LPS in murine peritoneal suppressor macrophages (MPSMs). It was found that IFN-gamma strongly enhanced the LPS-induced IL-12 p40 and p35 mRNA expression. Both p40 and p35 mRNA levels were approximately equal. IFN-gamma also greatly promoted the LPS-induced secretion of IL-12 p70 in MPSMs. The Proteasome Inhibitor I (PSI) could block the expression of IL-12 p40 and p35 mRNA, and the degradation of I kappaB alpha induced by LPS or LPS/IFN-gamma. EMSA showed that LPS could augment the NF-kappaB binding activity to p40 promoter DNA. However, IFN-gamma could neither enhance the LPS-induced NF-kappaB activity nor promote the degradation of I kappaB alpha. Taken together, the data suggest: (i) IFN-gamma /LPS could strongly induce the expression of IL-12 p40 and p35 mRNA; both the expression levels were equal; this phenomenon coincided with the high-level secretion of IL-12 p70 induced by IFN-gamma /LPS; (ii) NF-kappaB signal pathway is essential for IFN-gamma /LPS to induce IL-12 mRNA expression; (iii) by blocking the degradation of I kappaB, the PSI suppresses the IL-12 p40/p35 mRNA expression induced by LPS and IFN-gamma /LPS; (iv) NF-kappaB signal may not be involved in the mechanism by which IFN-gamma enhanced the expression of the LPS-induced IL-12 p40/p35 mRNA. |
| 学科主题 | Life Sciences & Biomedicine - Other Topics |
| 类目[WOS] | Biology |
| 关键词[WOS] | LIPOPOLYSACCHARIDE ; CELLS ; GENES ; DNA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000166396000003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2742]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ql, J,Zhang, JS,Feng, WG,et al. Promoting effect of IFN-gamma on the expression of LPS-induced IL-12 p40 and p35 mRNA in murine suppressor macrophages[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2000,43(6):578-588. |
| APA | Ql, J,Zhang, JS,Feng, WG,Luo, XD,Li, CL,&Zhang, ZL.(2000).Promoting effect of IFN-gamma on the expression of LPS-induced IL-12 p40 and p35 mRNA in murine suppressor macrophages.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,43(6),578-588. |
| MLA | Ql, J,et al."Promoting effect of IFN-gamma on the expression of LPS-induced IL-12 p40 and p35 mRNA in murine suppressor macrophages".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 43.6(2000):578-588. |
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