Synthesis of human proinsulin C-peptide and preparation of specific antibody to it
文献类型：期刊论文
| 作者 | Li, MY; Liu, ZH; Shao, XX; Xia, QC; Zhou, GM; Lin, QS; Cui, DF |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2000 |
| 卷号 | 32期号:6页码:665-668 |
| 关键词 | human C-peptide peptide synthesis oligo-peptide antigen |
| 通讯作者 | , |
| 英文摘要 | A HPLC and CE pure human proinsulin C-peptide was synthesized by solid-phase method and TSK column purification. Its amino acid sequence and MS were consistent with theoretical values. In comparison with the formly reported chemical synthesis of C-peptide, this method has the advantage of simplicity and higher overall yield (41%). To improve the immunogenicity and specificity of oligopeptide antibody, the acrylyl-C-peptides were transformed into a polymer; the product had a poly-propionyl-core matrix with C-peptide branches. This treatment gave a macromolecule with a M-r about 25 kD. By using the polymer to immunize New Zealand rabbits for 30 days, specific antiserum was obtained with titer of 2.5 x 10(4) (by ELISA), which did not cross react with BSA. Thus, the poly-propionyl-peptide system provided a new approach for preparing synthetic peptide antibody and therefore is promising for the preparation of synthetic peptide-based vaccine. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000165453600023 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2743]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, MY,Liu, ZH,Shao, XX,et al. Synthesis of human proinsulin C-peptide and preparation of specific antibody to it[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2000,32(6):665-668. |
| APA | Li, MY.,Liu, ZH.,Shao, XX.,Xia, QC.,Zhou, GM.,...&Cui, DF.(2000).Synthesis of human proinsulin C-peptide and preparation of specific antibody to it.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,32(6),665-668. |
| MLA | Li, MY,et al."Synthesis of human proinsulin C-peptide and preparation of specific antibody to it".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 32.6(2000):665-668. |
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