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Structural studies of the glycopeptides of B-chain of cinnamomin - a type II ribosome-inactivating protein by nuclear magnetic resonance
文献类型：期刊论文
| 作者 | Pu, Z; Li, YM; Hou, FJ; He, FH; Zhang, NX; Wu, HM; Liu, WY |
| 刊名 | GLYCOCONJUGATE JOURNAL
 |
| 出版日期 | 2000 |
| 卷号 | 17期号:11页码:749-759 |
| 关键词 | cinnamomin glycopeptides NMR primary structure ribosome-inactivating protein |
| 通讯作者 | Wu, HM (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Cinnamomin is a plant type II ribosome-inactivating protein (RIP) isolated from the seeds of Cinnamomum camphora. It consists of two nonidentical polypeptide chains (A- and B-chain) held together through one disulfide linkage. Its A- and B-chain contain 0.3% and 3.9% sugars respectively. The B-chain of cinnamomin was digested by pronase E and then the liberated glycopeptides were separated from non-glycopeptides by gel filtration chromatography on a Bio-Gel P-4 column. Three crude glycopeptides were obtained by continuing chromatography over anion-exchange resin (AG1-X2) in the buffer of 2% pyridine-acetic acid (pH 8.3) with a polygradient elution system. Through further purification by the gel filtration chromatography and HPLC, three major glycopeptides, GP1, GP2 and GP3 were obtained. Mainly by two-dimensional Nuclear Magnetic Resonance (NMR) including TOCSY, DQF-COSY, NOESY, HMQC and HMBC, their primary structures were analyzed as: Man alpha1,3Man alpha1,6(Man alpha1,3)(Xyl beta1,2)Man beta1,4GlcNAc beta1,4GlcNAc beta1-(Gly-)Asn-Asn-Thr(GP1), Man alpha1,6(Man alpha1,3)(Xyl beta1,2)Man beta1,4GlcNAc beta1,4(Fuc alpha1,3)GlcNAc beta1-Asn-Ala-Thr(GP2),Man alpha1,6(Man alpha1,3)Man alpha1,6(Man alpha1,2 Man alpha1,3)Man beta1,4GlcNAc beta1,4GlcNAc beta1-(Ala-)Asn-Gly-Thr(GP3). |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | N-LINKED OLIGOSACCHARIDES ; SUGAR CHAINS ; RICIN-D ; GLYCOPROTEINS ; SEEDS ; LECTINS ; XYLOSE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000169741000002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2744]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Pu, Z,Li, YM,Hou, FJ,et al. Structural studies of the glycopeptides of B-chain of cinnamomin - a type II ribosome-inactivating protein by nuclear magnetic resonance[J]. GLYCOCONJUGATE JOURNAL,2000,17(11):749-759. |
| APA | Pu, Z.,Li, YM.,Hou, FJ.,He, FH.,Zhang, NX.,...&Liu, WY.(2000).Structural studies of the glycopeptides of B-chain of cinnamomin - a type II ribosome-inactivating protein by nuclear magnetic resonance.GLYCOCONJUGATE JOURNAL,17(11),749-759. |
| MLA | Pu, Z,et al."Structural studies of the glycopeptides of B-chain of cinnamomin - a type II ribosome-inactivating protein by nuclear magnetic resonance".GLYCOCONJUGATE JOURNAL 17.11(2000):749-759. |
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