中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Femtosecond laser-assisted microinjection into living neurons

文献类型:期刊论文

作者Lei, Ming1; Xu, Hanpeng2; Yang, Hao2; Yao, Baoli1
刊名journal of neuroscience methods
出版日期2008-09-30
卷号174期号:2页码:215-218
关键词Microinjection Transfection Femtosecond laser Neuron
英文摘要a ti:sapphire femtosecond laser micro-manipulation system was used to perforate vital cells. propidium iodide was successfully transferred into both astrocytes and pc12 cells via the channel of a femtosecond laser, which induced transiently recoverable opening of the cell membrane. the transfection efficiency reached almost 100%. this method also had the advantage of contact-free, non-disruptive and stable transfection. (c) 2008 elsevier b.v. all rights reserved.
WOS标题词science & technology ; life sciences & biomedicine
类目[WOS]biochemical research methods ; neurosciences
研究领域[WOS]biochemistry & molecular biology ; neurosciences & neurology
关键词[WOS]cells ; transfection ; dna
收录类别SCI
语种英语
WOS记录号WOS:000259905600007
公开日期2015-08-31
源URL[http://ir.opt.ac.cn/handle/181661/25212]  
专题西安光学精密机械研究所_瞬态光学技术国家重点实验室
作者单位1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China
2.Fourth Mil Med Univ, Inst Neurosci, Xian 710032, Peoples R China
推荐引用方式
GB/T 7714
Lei, Ming,Xu, Hanpeng,Yang, Hao,et al. Femtosecond laser-assisted microinjection into living neurons[J]. journal of neuroscience methods,2008,174(2):215-218.
APA Lei, Ming,Xu, Hanpeng,Yang, Hao,&Yao, Baoli.(2008).Femtosecond laser-assisted microinjection into living neurons.journal of neuroscience methods,174(2),215-218.
MLA Lei, Ming,et al."Femtosecond laser-assisted microinjection into living neurons".journal of neuroscience methods 174.2(2008):215-218.

入库方式: OAI收割

来源:西安光学精密机械研究所

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