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Reactive carbonyl compounds (RCCs) cause aggregation and dysfunction of fibrinogen

文献类型：期刊论文

作者Xu, Ya-Jie1; Qiang, Min1,2; Zhang, Jin-Ling1; Liu, Ying1; He, Rong-Qiao1,3
刊名PROTEIN & CELL
出版日期2012-08-01
卷号3期号:8页码:627-640
关键词fibrinogen acrolein glycolaldehyde glyoxal malondialdehyde methylglyoxal
ISSN号1674-800X
产权排序1,3
通讯作者He, RQ (reprint author), Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China.
英文摘要Fibrinogen is a key protein involved in coagulation and its deposition on blood vessel walls plays an important role in the pathology of atherosclerosis. Although the causes of fibrinogen (fibrin) deposition have been studied in depth, little is known about the relationship between fibrinogen deposition and reactive carbonyl compounds (RCCs), compounds which are produced and released into the blood and react with plasma protein especially under conditions of oxidative stress and inflammation. Here, we investigated the effect of glycolaldehyde on the activity and deposition of fibrinogen compared with the common RCCs acrolein, methylglyoxal, glyoxal and malondialdehyde. At the same concentration (1 mmol/L), glycolaldehyde and acrolein had a stronger suppressive effect on fibrinogen activation than the other three RCCs. Fibrinogen aggregated when it was respectively incubated with glycolaldehyde and the other RCCs, as demonstrated by SDS-PAGE, electron microscopy and intrinsic fluorescence intensity measurements. Staining with Congo Red showed that glycolaldehyde-and acrolein-fibrinogen distinctly formed amyloid-like aggregations. Furthermore, the five RCCs, particularly glycolaldehyde and acrolein, delayed human plasma coagulation. Only glycolaldehyde showed a markedly suppressive effect on fibrinogenesis, none did the other four RCCs when their physiological blood concentrations were employyed, respectively. Taken together, it is glycolaldehyde that suppresses fibrinogenesis and induces protein aggregation most effectively, suggesting a putative pathological process for fibrinogen (fibrin) deposition in the blood.
学科主题Physiological psychology/Biological Psychology
收录类别SCI
项目简介We thank Dr. Joy Fleming for helpful comments and improving the English of the manuscript. This work was supported by the National Basic Research Program (973 Program) (Grant Nos. 2010CB912303 and 2012CB911004), and the Natural Scientific Foundation of China (Grant Nos. NSFC 30970695 and QBI-IBP GJHZ1131).
原文出处http://library.ibp.ac.cn/html/slwj/000310531900009.pdf
语种英语
WOS记录号WOS:000310531900009
源URL[http://ir.psych.ac.cn/handle/311026/12909]  
专题心理研究所_脑与认知科学国家重点实验室
作者单位1.Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
推荐引用方式
GB/T 7714		 Xu, Ya-Jie,Qiang, Min,Zhang, Jin-Ling,et al. Reactive carbonyl compounds (RCCs) cause aggregation and dysfunction of fibrinogen[J]. PROTEIN & CELL,2012,3(8):627-640.
APA Xu, Ya-Jie,Qiang, Min,Zhang, Jin-Ling,Liu, Ying,&He, Rong-Qiao.(2012).Reactive carbonyl compounds (RCCs) cause aggregation and dysfunction of fibrinogen.PROTEIN & CELL,3(8),627-640.
MLA Xu, Ya-Jie,et al."Reactive carbonyl compounds (RCCs) cause aggregation and dysfunction of fibrinogen".PROTEIN & CELL 3.8(2012):627-640.

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来源：心理研究所

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