Characterization of complexation of PVP copolymer with DNA
文献类型：期刊论文
| 作者 | Zhang, Lifen ; Liang, Yong ; Meng, Lingzhi ; Wang, Chang |
| 刊名 | POLYMERS FOR ADVANCED TECHNOLOGIES
 |
| 出版日期 | 2009-04 |
| 卷号 | 20期号:4页码:410-415 |
| 关键词 | PVP copolymer DNA interaction amphiphiles fluorescence water-soluble polymer |
| 中文摘要 | Interactions of amphiphilic copolymer poly(N-vinylpyrrolidone-co-N-t-Boc-tryptophanamido-N'-methacrylthiourea) [P(NVP-co-TrpAMT)] and poly(N-vinylpyrrlidone) (PVP) with DNA were studied with the aim to understand the difference in their complexation efficiencies when considered as a potential non-viral delivery vector. Fluorescence spectroscopy, ethidium bromide (EB) quenching, zeta potential measurement, and agarose gel electrophoresis were all carried out in Tris-HCl (pH 7.4) solution to assess the degree of interaction between the (co)polymers P(NVP-co-TrpAMT) and DNA. All of these measurements show that the high affinity of the copolymer for DNA can be demonstrated. We also found that P(NVP-co-TrpAMT)-I (PVP/PTrpAMT molar ratio was 100:2.88) exhibited a high DNA cleavage activity and induced the supercoiled form of the plasmid cleavage in the nicked and linear forms. Moreover, P(NVP-co-TrpAMT)-I exhibited stronger affinity for DNA than PVP and P(NVP-co-TrpAMT)-II (PVP/PTrpAMT molar ratio of 100:6.35). This phenomenon is believed to be related to the structure of TrpAMT and the hydrophobic nature of the copolymer. P(NVP-co-TrpAMT)-II with higher hydrophobic segments creates a strong hydrophobic interaction and induces a steric barrier which hinders their complexation with DNA. The study reveals important information about the inner mechanisms involved in the interaction of the PVP copolymer with DNA, which aids in the design of novel efficient non-ionic gene carriers. Copyright (C) 2008 John Wiley & Sons, Ltd. |
| WOS记录号 | WOS:000264948300008 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21468]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang, Lifen,Liang, Yong,Meng, Lingzhi,et al. Characterization of complexation of PVP copolymer with DNA[J]. POLYMERS FOR ADVANCED TECHNOLOGIES,2009,20(4):410-415. |
| APA | Zhang, Lifen,Liang, Yong,Meng, Lingzhi,&Wang, Chang.(2009).Characterization of complexation of PVP copolymer with DNA.POLYMERS FOR ADVANCED TECHNOLOGIES,20(4),410-415. |
| MLA | Zhang, Lifen,et al."Characterization of complexation of PVP copolymer with DNA".POLYMERS FOR ADVANCED TECHNOLOGIES 20.4(2009):410-415. |
入库方式： OAI收割
来源：生态环境研究中心
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